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Titolo:
Effects of beta-naphthoflavone on the cytochrome P450 system, and phase IIenzymes in gilthead seabream (Sparus aurata)
Autore:
Pretti, C; Salvetti, A; Longo, V; Giorgi, M; Gervasi, PG;
Indirizzi:
Univ Pisa, Dipartimento Patol Anim Profilassi Igiene Aliment, I-56124 Pisa, Italy Univ Pisa Pisa Italy I-56124 filassi Igiene Aliment, I-56124 Pisa, Italy Univ Pisa, Dipartimento Morfol Umana & Biol Applicata, I-56124 Pisa, ItalyUniv Pisa Pisa Italy I-56124 Umana & Biol Applicata, I-56124 Pisa, Italy CNR, Ist Mutagenesi & Differenziamento, Lab Genet & Biochim Tossicol, I-56124 Pisa, Italy CNR Pisa Italy I-56124 Lab Genet & Biochim Tossicol, I-56124 Pisa, Italy Univ Pisa, Dipartimento Clin Vet, I-56124 Pisa, Italy Univ Pisa Pisa Italy I-56124 Dipartimento Clin Vet, I-56124 Pisa, Italy
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
fascicolo: 1, volume: 130, anno: 2001,
pagine: 133 - 144
SICI:
1532-0456(200109)130:1<133:EOBOTC>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
TROUT ONCORHYNCHUS-MYKISS; BASS DICENTRARCHUS-LABRAX; 2 TELEOST FISH; RAINBOW-TROUT; BIOTRANSFORMATION ENZYMES; DT-DIAPHORASE; RAT-LIVER; HEPATIC BIOTRANSFORMATION; MONOOXYGENASE ACTIVITY; ANTIOXIDANT ENZYMES;
Keywords:
CYP 1A; Sparus aurata; beta-naphtoflavone; DT-diaphorase; aldehyde dehydrogenase; glutathione S-transferase; UDP-glucuronyl transferase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Pretti, C Univ Pisa, Dipartimento Patol Anim Profilassi Igiene Aliment, Vle Piagge 2, I-56124 Pisa, Italy Univ Pisa Vle Piagge 2 Pisa Italy I-56124 , I-56124 Pisa, Italy
Citazione:
C. Pretti et al., "Effects of beta-naphthoflavone on the cytochrome P450 system, and phase IIenzymes in gilthead seabream (Sparus aurata)", COMP BIOC C, 130(1), 2001, pp. 133-144

Abstract

The effect of P-naphthoflavone (P-NF) on several catalytic activities of cytochrome P450 (CYP) and phase II enzymes putatively controlled by [Ah]-receptor activation in the liver, heart and kidney of gilthead seabream, was investigated. In the liver, P-NF treatment [intraperitoneal injection (i.p.)50 mg/kg] resulted in an increase of CYP content, immunoreactive CYP IA and methoxyresorufin-O-demethylase (MEROD), pentoxyresorufin O-depentylase (PROD) and ethoxyresorufin-O-deethylase (EROD) activities. However, beta -NF had no effect on any of the hepatic phase II enzymes examined (benzaldehydedehydrogenase, propionaldehyde dehydrogenase, glutathione S-transferase, UDP-glucuronyl-transferase, DT-diaphorase). Single i.p. injection of 10 mg/kg beta -NF showed a maximal induction of CYP 1A-like protein and EROD activity after 3-7 days. CYP 1A and EROD returned to control levels 18-days post-treatment. beta -NF injection also caused a rapid increase of a single band size of mRNA recognized by a CYP 1A1 cDNA fragment from sea bass (Dicentrarchus labrax). Expression of mRNA preceded the increase of EROD activity and declined rapidly by 96 h. Dose-response experiments demonstrated that EROD was significantly enhanced in liver by a single injection of 0.3 mg/kg beta -NF and was the most sensitive measurement for CYP IA-like induction. P-NF treatments also increased the expression of CYP IA-like protein, mRNA and EROD, but not MEROD and PROD activities in heart and kidney, (C) 2001 Published by Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 10:10:24