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Titolo:
Catalytic and immunochemical properties of hepatic cytochrome P450 1A in three avian species treated with beta-naphthoflavone or isosafrole
Autore:
Verbrugge, LA; Giesy, JP; Verbrugge, DA; Woodin, BR; Stegeman, JJ;
Indirizzi:
Michigan State Univ, Natl Food Safety & Toxicol Ctr, Inst Environm Toxicol, Dept Zool, E Lansing, MI 48824 USA Michigan State Univ E Lansing MI USA 48824 Zool, E Lansing, MI 48824 USA Michigan State Univ, Dept Fisheries & Wildlife, Pesticide Res Ctr, E Lansing, MI 48824 USA Michigan State Univ E Lansing MI USA 48824 s Ctr, E Lansing, MI 48824 USA Michigan State Univ, Inst Environm Toxicol, E Lansing, MI 48824 USA Michigan State Univ E Lansing MI USA 48824 xicol, E Lansing, MI 48824 USA Woods Hole Oceanog Inst, Dept Biol, Woods Hole, MA 02543 USA Woods Hole Oceanog Inst Woods Hole MA USA 02543 Woods Hole, MA 02543 USA
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
fascicolo: 1, volume: 130, anno: 2001,
pagine: 67 - 83
SICI:
1532-0456(200109)130:1<67:CAIPOH>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAFFEINE BREATH TEST; DIBENZO-P-DIOXINS; 2 TELEOST FISH; STENOTOMUS-CHRYSOPS; HEPATOCYTE CULTURES; LIVER-MICROSOMES; CROSS-REACTIVITY; ENVIRONMENTAL CONTAMINATION; POLYCHLORINATED-BIPHENYLS; CHICK-EMBRYO;
Keywords:
alkoxyresorufin-O-dealkylase; beta-naphthoflavone; chicken; cytochrome P450 1A; double-crested cormorant; Herring Gull; immunochemistry; isosafrole; furafylline; ellipticine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
63
Recensione:
Indirizzi per estratti:
Indirizzo: Giesy, JP Michigan State Univ, Natl Food Safety & Toxicol Ctr, Inst Environm Toxicol, Dept Zool, E Lansing, MI 48824 USA Michigan State Univ E Lansing MI USA 48824 ansing, MI 48824 USA
Citazione:
L.A. Verbrugge et al., "Catalytic and immunochemical properties of hepatic cytochrome P450 1A in three avian species treated with beta-naphthoflavone or isosafrole", COMP BIOC C, 130(1), 2001, pp. 67-83

Abstract

Induction of cytochrome P450 IA (CYP1A) can be used as a biomarker of exposure to planar halogenated aromatic hydrocarbons (PHAHs). Our objective wasto characterize the induction of CYP1A activity and protein in three avianspecies following in vivo treatment with beta -naphthoflavone (BNF) and/orisosafrole. Alkoxyresorufin-O-dealkylase (alk-ROD) activities of hepatic microsomes from Herring Gulls (Larus argentatus) (HGs), Double-crested Cormorants (Phalacrocorax auritus) (DCCs) and chickens (Gallus domesticus) were measured using ethoxy-, methoxy-, pentoxy- and benzyloxy-resorufin, in the presence and absence of the inhibitors ellipticine or furafylline. Immunoreactivity of microsomal proteins with antibodies to several CYP1A proteins was investigated. CYP1A protein and alk-ROD activities of HGs and DCCs. but not chickens, were induced by isosafrole. Ellipticine was a potent and non-selective inhibitor of alk-ROD activity in all three species, while furafylline inhibition of alk-ROD activities varied among species and treatments. In all three species, BNF induced a protein immunoreactive with monoclonal antibody to CYP1A1 from the marine fish Stenotomus chrysops (scup), but a CYP1A2-like protein was not detected in avian microsomes probed with polyclonal antibodies to mouse CYP1A2. Variations in responses among avian speciesindicate that CYP1A proteins and substrate specificities should be characterized for each species used in PHAH biomonitoring programs. (C) 2001 Published by Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 18:36:39