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Titolo:
Activation of Raf/ERK1/2 MAP kinase pathway is involved in GM-CSF-induced proliferation and survival but not in erythropoietin-induced differentiation of TF-1 cells
Autore:
Kolonics, A; Apati, A; Janossy, J; Brozik, A; Gati, R; Schaefer, A; Magocsi, M;
Indirizzi:
Natl Inst Haematol & Immunol, Dept Cell Metab, H-1113 Budapest, Hungary Natl Inst Haematol & Immunol Budapest Hungary H-1113 3 Budapest, Hungary Hungarian Acad Sci, Inst Enzymol, Budapest, Hungary Hungarian Acad Sci Budapest Hungary ci, Inst Enzymol, Budapest, Hungary Univ Hamburg, Sch Med, Dept Toxicol, D-2000 Hamburg, Germany Univ HamburgHamburg Germany D-2000 ept Toxicol, D-2000 Hamburg, Germany
Titolo Testata:
CELLULAR SIGNALLING
fascicolo: 10, volume: 13, anno: 2001,
pagine: 743 - 754
SICI:
0898-6568(200110)13:10<743:AORMKP>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; TERNARY COMPLEX FACTORS; INDUCED ERYTHROID-DIFFERENTIATION; MURINE ERYTHROLEUKEMIA-CELLS; C-FOS EXPRESSION; MEGAKARYOCYTIC DIFFERENTIATION; SIGNALING PATHWAY; INDUCED APOPTOSIS; TRANSCRIPTIONAL ACTIVATION; HEMATOPOIETIC-CELLS;
Keywords:
erythropoietin; GM-CSF; TF-1 cells; ERKs; signal transduction; differentiation; cell proliferation; apoptosis; transcription factors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
81
Recensione:
Indirizzi per estratti:
Indirizzo: Magocsi, M Natl Inst Haematol & Immunol, Dept Cell Metab, Daroczi Ut 24, H-1113 Budapest, Hungary Natl Inst Haematol & Immunol Daroczi Ut 24 Budapest Hungary H-1113
Citazione:
A. Kolonics et al., "Activation of Raf/ERK1/2 MAP kinase pathway is involved in GM-CSF-induced proliferation and survival but not in erythropoietin-induced differentiation of TF-1 cells", CELL SIGNAL, 13(10), 2001, pp. 743-754

Abstract

The involvement of MAPK pathways in differentiation, proliferation and survival was investigated by comparing Epo and GM-CSF signalling in human factor-dependent myeloerythroid TF-1 cells with abnormal Epo-R. GM-CSF withdrawal induced cell-cycle arrest and apoptosis accompanied by increased caspase-3 activity, DNA degradation and reduced expression of the antiapoptotic Bcl-2 and Bcl-xl proteins. Readministration of GM-CSF but act Epo reversed these processes and induced proliferation. The GM-CSF promoted cell survival and proliferation correlated with MEK-1 dependent ERK1/2, Elk-1 and CREB phosphorylation and Egr-1, c-Fos expression as well as with increased STAT-5,AP-1, c-Myb and NF-kappaB DNA-binding. In contrast, Epo failed to activatethe Raf-1/ERK1/2 MAPK pathway or to induce Egr-1 and/or c-Fos expression, while it induced erythroid differentiation in GM-CSF-deprived cells. In addition, the Epo-induced haemoglobin production was inhibited in the presenceof GM-CSF. These results demonstrate that the activation of MAPK cascade is not necessary for Epo-induced haemoglobin production in TF-1 cells and suggest a negative cross-talk between the signalling of GM-CSF-stimulated cell proliferation and Epo-induced erythroid differentiation. (C) 2001 Elsevier Science Inc. All rights reserved.

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Documento generato il 02/04/20 alle ore 19:12:21