Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Detection of tumor cells in the portal and peripheral blood of patients with colorectal carcinoma using competitive reverse transcriptase-polymerase chain reaction
Autore:
Sadahiro, S; Suzuki, T; Tokunaga, N; Yurimoto, S; Yasuda, S; Tajima, T; Makuuchi, H; Murayama, C; Matsuda, K;
Indirizzi:
Tokai Univ, Sch Med, Dept Surg, Isehara, Kanagawa 2591193, Japan Tokai Univ Isehara Kanagawa Japan 2591193 sehara, Kanagawa 2591193, Japan Mitsubishi Bioclin Labs Inc, Dept Genet, Tokyo, Japan Mitsubishi Bioclin Labs Inc Tokyo Japan s Inc, Dept Genet, Tokyo, Japan
Titolo Testata:
CANCER
fascicolo: 5, volume: 92, anno: 2001,
pagine: 1251 - 1258
SICI:
0008-543X(20010901)92:5<1251:DOTCIT>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
CIRCULATING MALIGNANT CELLS; TOUCH ISOLATION TECHNIQUE; MOLECULAR-DETECTION; CANCER PATIENTS; BONE-MARROW; LYMPH-NODES; PROGNOSTIC-SIGNIFICANCE; COLON; RESECTION; SURGERY;
Keywords:
colorectal carcinoma; carcinoembryonic antigen (CEA); circulating tumor cells; reverse transcriptase-polymerase chain reaction (RT-PCR);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Sadahiro, S Tokai Univ, Sch Med, Dept Surg, Isehara, Kanagawa 2591193, Japan Tokai Univ Isehara Kanagawa Japan 2591193 gawa 2591193, Japan
Citazione:
S. Sadahiro et al., "Detection of tumor cells in the portal and peripheral blood of patients with colorectal carcinoma using competitive reverse transcriptase-polymerase chain reaction", CANCER, 92(5), 2001, pp. 1251-1258

Abstract

BACKGROUND. in spite of many reports, it remains unclear whether the presence of tumor cells in circulating blood flow predicts a poor prognosis. METHODS. Competitive seminested reverse transcriptase-polymerase chain reaction (RT-PCR), a technique for the quantitative detection of tumor cells, was applied to detect the presence of tumor cells in portal and peripheral blood samples from 121 patients with colorectal carcinoma and to clarify their clinical significance. This technique can detect one carcinoembryonic antigen (CEA) mRNA-expressing tumor cell in 1 x 10(5) normal lymphocytes. RESULTS. Six of 33 healthy volunteers (18%) demonstrated a positive reaction to this technique. CEA mRNA expression was detected in the portal blood in 51% of patients and in the peripheral blood in 42% of patients. The results from the two blood samples were consistent in 91% of patients. The positive expression rates for portal blood in patients with T1 tumors and thosewith TNM Stage I disease were 38% and 45%, respectively. The positive ratewas significantly higher in patients with colon carcinoma and those with Stage III or IV disease. CEA mRNA expression, quantitatively measured (x 10(-8)/beta -actin), was 22.9 +/- 35.1 in the portal blood and 19.9 +/- 40.0 in the peripheral blood, with no statistically significant difference. A significant positive correlation was noted between portal and peripheral CEA mRNA expression levels according to Speaman correlation analysis (correlation coefficient = 0.78; P < 0.01). Multivariate analysis revealed that the positive rate and level of CFA mRNA expression in the portal and peripheral blood did not appear to be influenced by the established prognostic factors. CONCLUSIONS. The presence of circulating tumor cells might be of less value as a prognostic factor because they also can be detected in patients withearly-stage colorectal carcinoma and appeared to be independent of the conventional prognostic factors. (C) 2001 American Cancer Society.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 16:39:55