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Titolo:
Tumor necrosis factor (TNF)-mediated activation of the p55 TNF receptor negatively regulates maintenance of cycling reconstituting human hematopoietic stem cells
Autore:
Dybedal, I; Bryder, D; Fossum, A; Rusten, LS; Jacobsen, SEW;
Indirizzi:
Univ Lund Hosp, Inst Lab Med, Dept Stem Cell Biol, S-22185 Lund, Sweden Univ Lund Hosp Lund Sweden S-22185 Stem Cell Biol, S-22185 Lund, Sweden Norwegian Radium Hosp, Dept Oncol, Oslo, Norway Norwegian Radium Hosp Oslo Norway Radium Hosp, Dept Oncol, Oslo, Norway
Titolo Testata:
BLOOD
fascicolo: 6, volume: 98, anno: 2001,
pagine: 1782 - 1791
SICI:
0006-4971(20010915)98:6<1782:TNF(AO>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
EX-VIVO EXPANSION; HUMAN CORD-BLOOD; PROGENITOR CELLS; BONE-MARROW; IN-VITRO; TERM-CULTURE; FACTOR-ALPHA; INTERFERON-GAMMA; SELF-RENEWAL; C-MPL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Jacobsen, SEW Univ Lund Hosp, Inst Lab Med, Dept Stem Cell Biol, S-22185 Lund, Sweden Univ Lund Hosp Lund Sweden S-22185 l, S-22185 Lund, Sweden
Citazione:
I. Dybedal et al., "Tumor necrosis factor (TNF)-mediated activation of the p55 TNF receptor negatively regulates maintenance of cycling reconstituting human hematopoietic stem cells", BLOOD, 98(6), 2001, pp. 1782-1791

Abstract

Hematopoietic stem cell (HSC) fate decisions between self-renewal and commitment toward differentiation are tightly regulated in vivo. Recent developments in HSC culture and improvements of human HSC assays have facilitated studies of these processes in vitro. Through such studies stimulatory cytokines critically involved in HSC maintenance in vivo have been demonstrated to also promote HSC self-renewing divisions in vitro. Evidence for negativeregulators of HSC self-renewal is, however, lacking. Tumor necrosis factor(TNF), if overexpressed, has been implicated to mediate bone marrow suppression. However, whether and how TNF might affect the function of HSC with acombined myeloid and lymphoid reconstitution potential has not been investigated. In the present studies in vitro conditions recently demonstrated topromote HSC self-renewing divisions in vitro were used to study the effectof TNF on human HSCs capable of reconstituting myelopoiesis and lymphopoiesis in nonobese diabetic-severe combined immunodeficient (NOD-SCID) mice. Although all cord blood and adult bone marrow CD34(+)CD38(-) cells were capable of undergoing cell divisions in the presence of TNF, cycling HSCs exposed to TNF in vitro and in vivo were severely compromised in their ability to reconstitute NOD-SCID mice and longterm cultures. The negative effect of TNF was not dependent on the Fas pathway, and a similar effect could be observed using a mutant TNF exclusively targeting the p55 TNF receptor. TNF did not appear to enhance apoptosis or affect cell-cycle distribution of cultured progenitors, but rather promoted myeloid differentiation. Thus, TNF might regulate HSC fate by promoting their differentiation rather than self-renewal. (C) 2001 by The American Society of Hematology

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 12:01:11