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Titolo:
Vascular endothelial growth factor-A and -C protein up-regulation and early angiogenesis in a rat photothrombotic ring stroke model with spontaneous reperfusion
Autore:
Gu, WG; Brannstrom, T; Jiang, W; Bergh, A; Wester, P;
Indirizzi:
Umea Univ, Dept Med, S-90187 Umea, Sweden Umea Univ Umea Sweden S-90187Umea Univ, Dept Med, S-90187 Umea, Sweden Umea Univ, Dept Pathol Neuropathol, S-90187 Umea, Sweden Umea Univ Umea Sweden S-90187 t Pathol Neuropathol, S-90187 Umea, Sweden Umea Univ, Dept Publ Hlth & Clin Med, Umea Stroke Ctr, Res Lab, S-90187 Umea, Sweden Umea Univ Umea Sweden S-90187 Stroke Ctr, Res Lab, S-90187 Umea, Sweden
Titolo Testata:
ACTA NEUROPATHOLOGICA
fascicolo: 3, volume: 102, anno: 2001,
pagine: 216 - 226
SICI:
0001-6322(200109)102:3<216:VEGFA->2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
MIDDLE CEREBRAL-ARTERY; ISCHEMIC STROKE; FACTOR EXPRESSION; VEGF RECEPTOR-1; DNA-SYNTHESIS; FACTOR-B; IN-VIVO; CELLS; BRAIN; HYPOXIA;
Keywords:
cerebral ischemia; photothrombosis; reperfusion; vascular endothelial growth factor; angiogenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Wester, P Umea Univ, Dept Publ Hlth & Clin Med, Umea Stroke Ctr, Res Lab, UKBF 5B, S-90187 Umea, Sweden Umea Univ UKBF 5B Umea Sweden S-90187 5B, S-90187 Umea, Sweden
Citazione:
W.G. Gu et al., "Vascular endothelial growth factor-A and -C protein up-regulation and early angiogenesis in a rat photothrombotic ring stroke model with spontaneous reperfusion", ACT NEUROP, 102(3), 2001, pp. 216-226

Abstract

This study explored the temporal expression pattern of two subtypes of vascular endothelial growth factor (VEGF) proteins and three subforms of theirreceptors as well as endothelial proliferation in adult rats subjected to photothrombotic ring stroke with spontaneous reperfusion in the cortical region at risk. The exposed crania of halothane-anesthetized, temperature- and blood gas-controlled male Wistar rats were irradiated with a ring laser beam started simultaneously with systemic injection of the photosensitizer erythrosin B. Rats were repeatedly injected with 5-bromodeoxyuridine (BrdU) after stroke induction. Immunohistochemistry of coronal brain sections showed that VEGF protein subtype C increased simultaneously with subtype A in the ring lesion region at 2 h after irradiation. In the cortical region at risk (i.e., the penumbra-like zone), increased VEGF-C and VEGF-A immunostaining was seen at 24 h with sustained appearance up to 72 h after ischemic onset. Correspondingly, the VEGF-C-specific receptor flt-4 and the VEGF-A receptors flt-1 and flk-1 were up-regulated in a temporal sequence similar to that of their agonist proteins in the cortical ring lesion and the region at risk. At 48 h after stroke induction, proliferating BrdU-immunopositive endothelial cells formed microvessels in the post-ischemic cortical region at risk. These vessels became more pronounced at 72 h and were still visibleat 100 days after the stroke. This study suggests that VEGF-C and its receptor flt-4 may cooperate with VEGF-A and its receptors flt-1 and flk-1 to promote early angiogenesis after stroke, which may in turn contribute to spontaneous reperfusion in this focal thromboembolic stroke model.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 18:20:05