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Titolo:
Plasma membrane resident 'fusion complexes' mediate reconstituted exocytosis
Autore:
Ikebuchi, Y; Baibakov, B; Smith, RM; Vogel, SS;
Indirizzi:
Med Coll Georgia, Dept Med, Inst Mol Med & Genet, Augusta, GA 30912 USA Med Coll Georgia Augusta GA USA 30912 Med & Genet, Augusta, GA 30912 USA
Titolo Testata:
TRAFFIC
fascicolo: 9, volume: 2, anno: 2001,
pagine: 654 - 667
SICI:
1398-9219(200109)2:9<654:PMR'CM>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEA-URCHIN EGG; INDIVIDUAL SECRETORY VESICLES; CALCIUM-TRIGGERED EXOCYTOSIS; CORTICAL-GRANULE EXOCYTOSIS; INVITRO RECONSTITUTION; SNARE HYPOTHESIS; ACTIVATION; RELEASE; SENSITIVITY; PROTEINS;
Keywords:
actin; cytochalasin D; endosome; microscopy; N-ethylmaleimide; sea urchin egg;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Vogel, SS Med Coll Georgia, Dept Med, Inst Mol Med & Genet, 1120 15th St,CB 2803, Augusta, GA 30912 USA Med Coll Georgia 1120 15th St,CB 2803 AugustaGA USA 30912 2 USA
Citazione:
Y. Ikebuchi et al., "Plasma membrane resident 'fusion complexes' mediate reconstituted exocytosis", TRAFFIC, 2(9), 2001, pp. 654-667

Abstract

Calcium-triggered exocytosis is thought to be mediated by membrane-associated protein complexes. In sea urchin eggs, high concentrations of calcium activate multiple 'fusion complexes' per cortical vesicle-plasma membrane docking site. Some of these fusion complexes are known to reside in the vesicle membrane. It is not known if fusion complexes also reside in the plasma membrane, or if plasma membrane-resident fusion complexes require cognate partners in the vesicle membrane. Using reconstitution, we show that N-ethylmaleimide treatment of either vesicles or plasma membrane fragments prior to reconstitution does not completely inhibit exocytosis. Treatment of both components did result in complete inhibition. Upon reconstitution, corticalvesicles and the early endosomes formed by compensatory endocytosis both contributed, on average, two fusion complexes per reconstituted docking site. The plasma membrane contributed, on average, two fusion complexes per docking site when assembled with cortical vesicles, but only one complex when reconstituted with endosomes. We conclude that there are at least two typesof plasma membrane-resident fusion complexes that participate in reconstituted cortical vesicle-plasma membrane fusion. The activity of one of these fusion complexes is target-specific for cortical vesicles, while the secondtype also supports fusion with endosomes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/21 alle ore 16:12:34