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Titolo:
Characterization of Salmonella-induced filaments (Sifs) reveals a delayed interaction between Salmonella-containing vacuoles and late endocytic compartments
Autore:
Brumell, JH; Tang, P; Mills, SD; Finlay, BB;
Indirizzi:
Univ British Columbia, Biotechnol Lab, Vancouver, BC V6T 1A3, Canada Univ British Columbia Vancouver BC Canada V6T 1A3 ver, BC V6T 1A3, Canada Univ British Columbia, Dept Biochem & Mol Biol, Vancouver, BC V6T 1A3, Canada Univ British Columbia Vancouver BC Canada V6T 1A3 ver, BC V6T 1A3, Canada Univ British Columbia, Dept Microbiol & Immunol, Vancouver, BC V6T 1A3, Canada Univ British Columbia Vancouver BC Canada V6T 1A3 ver, BC V6T 1A3, Canada
Titolo Testata:
TRAFFIC
fascicolo: 9, volume: 2, anno: 2001,
pagine: 643 - 653
SICI:
1398-9219(200109)2:9<643:COSF(R>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
LYSOSOMAL MEMBRANE-GLYCOPROTEINS; III SECRETION SYSTEM; TYPHIMURIUM-CONTAINING VACUOLES; PATHOGENICITY ISLAND 2; EPITHELIAL-CELLS; BACTERIAL INVASION; HOST-CELLS; VIRULENCE; PROTEIN; IDENTIFICATION;
Keywords:
endosome; lysosome; microbial pathogenesis; type III secretion;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Finlay, BB Univ British Columbia, Biotechnol Lab, Vancouver, BC V6T 1A3, Canada Univ British Columbia Vancouver BC Canada V6T 1A3 1A3, Canada
Citazione:
J.H. Brumell et al., "Characterization of Salmonella-induced filaments (Sifs) reveals a delayed interaction between Salmonella-containing vacuoles and late endocytic compartments", TRAFFIC, 2(9), 2001, pp. 643-653

Abstract

Salmonella typhimurium is a facultative intracellular pathogen that colonizes host cells throughout the course of infection. A unique feature of thispathogen is its ability to enter into (invade) epithelial cells and elongate the vacuole within which it resides into tubular structures called Salmonella-induced filaments (Sifs). In this study we sought to characterize themechanism of Sif formation by immunofluorescence analysis using subcellular markers. The late endosomal lipid lysobisphosphatidic acid associated in a punctate pattern with the Salmonella-containing vacuole, starting 90 min after infection and increasing thereafter. Lysobisphosphatidic acid-rich vesicles were also found to interact with Sifs, at numerous sites along the tubules. Similarly, cholesterol-rich vesicles were also found in associationwith intracellular bacteria and Sifs. The lysosomal hydrolase cathepsin D was present in Sifs, both in a punctate pattern and, at later times, predominantly in an uninterrupted linear pattern. Rab7 associated with Sifs and expression of the N1251 dominant negative mutant of this GTPase inhibited Sif formation. Transfection of HeLa cells with a vector encoding SifA fused to the green fluorescent protein caused swelling and aggregation of lysobisphosphatidic acid-containing compartments, suggesting that this virulence factor directs membrane fusion events involving late endosomes. Our findings demonstrate that Sif formation involves fusion of late endocytic compartments with the Salmonella-containing vacuole, and suggest that SifA modulates this event.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 18:29:28