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Titolo:
L-tyrosine and nitric oxide synergize to prevent cytotoxic effects of superoxide
Autore:
Souici, AC; Fitzhugh, AL; Keefer, LK; Felley-Bosco, E;
Indirizzi:
Inst Pharmacol & Toxicol, CH-1005 Lausanne, Switzerland Inst Pharmacol & Toxicol Lausanne Switzerland CH-1005 sanne, Switzerland NCI, Intramural Res Support Program, SAIC, Frederick, MD 21702 USA NCI Frederick MD USA 21702 Support Program, SAIC, Frederick, MD 21702 USA NCI, Chem Sect, Comparat Carcinogenesis Lab, Frederick, MD 21702 USA NCI Frederick MD USA 21702 at Carcinogenesis Lab, Frederick, MD 21702 USA
Titolo Testata:
TOXICOLOGY
fascicolo: 2-3, volume: 165, anno: 2001,
pagine: 163 - 170
SICI:
0300-483X(20010828)165:2-3<163:LANOST>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
HYDROGEN-PEROXIDE; XANTHINE-OXIDASE; PEROXYNITRITE FORMATION; LIPID-PEROXIDATION; PHYSIOLOGICAL PH; IN-VIVO; NITRATION; CELLS; MACROPHAGES; OXYGEN;
Keywords:
nitric oxide; reactive oxygen species; reactive nitrogen species; L-tyrosine; epithelial cells; oxidative damage;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Felley-Bosco, E Inst Pharmacol & Toxicol, Bugnon 27, CH-1005 Lausanne, Switzerland Inst Pharmacol & Toxicol Bugnon 27 Lausanne Switzerland CH-1005
Citazione:
A.C. Souici et al., "L-tyrosine and nitric oxide synergize to prevent cytotoxic effects of superoxide", TOXICOLOGY, 165(2-3), 2001, pp. 163-170

Abstract

We found previously that the nitric oxide donor DEA/NO enhanced lipid peroxidation, DNA fragmentation, and cytotoxicity in human bronchial epithelialcells (BEAS-2B) when they were cultured in LHC-8 medium containing the superoxide-generating system hypoxanthine/xanthine oxidase (HX/XO). We have now discovered that DEA/NO's prooxidant action can be reversed by raising theL-tyrosine concentration from 30 to 400 muM. DEA/NO also protected the cells when they were cultured in Dulbecco's Modified Eagle's Medium (DMEM), whose standard concentration Of L-tyrosine is 400 muM. Similar trends were seen with the colon adenoma cell line CaCo-2. Since HPLC analysis of cell-free DMEM or LHC-8 containing 400 PM L-tyrosine, DEA/NO, and HX/XO revealed noevidence of L-tyrosine nitration, our data suggest the existence of an as-yet uncharacterized mechanism by which L-tyrosine can influence the biochemical and toxicological effects of reactive nitrogen species. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 22:19:28