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Titolo:
Monoclonal anti-double stranded DNA antibody is a leucocyte-binding protein to up-regulate interleukin-8 gene expression and elicit apoptosis of normal human polymorphonuclear neutrophils
Autore:
Hsieh, SC; Sun, KH; Tsai, CY; Tsai, YY; Tsai, ST; Huang, DF; Han, SH; Yu, HS; Yu, CL;
Indirizzi:
Natl Yang Ming Univ, Sch Med, Inst Clin Med, Taipei 112, Taiwan Natl Yang Ming Univ Taipei Taiwan 112 Inst Clin Med, Taipei 112, Taiwan Natl Yang Ming Univ, Fac Med Technol, Taipei 112, Taiwan Natl Yang Ming Univ Taipei Taiwan 112 ac Med Technol, Taipei 112, Taiwan Vet Gen Hosp, Dept Med, Taipei, Taiwan Vet Gen Hosp Taipei TaiwanVet Gen Hosp, Dept Med, Taipei, Taiwan Kaohsiung Med Univ, Dept Dermatol, Kaohsiung, Taiwan Kaohsiung Med Univ Kaohsiung Taiwan v, Dept Dermatol, Kaohsiung, Taiwan Natl Taiwan Univ, Coll Med, Dept Med, Taipei, Taiwan Natl Taiwan Univ Taipei Taiwan Univ, Coll Med, Dept Med, Taipei, Taiwan Natl Taiwan Univ, Coll Med, Inst Mol Med, Taipei, Taiwan Natl Taiwan UnivTaipei Taiwan , Coll Med, Inst Mol Med, Taipei, Taiwan
Titolo Testata:
RHEUMATOLOGY
fascicolo: 8, volume: 40, anno: 2001,
pagine: 851 - 858
SICI:
1462-0324(200108)40:8<851:MASDAI>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYSTEMIC-LUPUS-ERYTHEMATOSUS; GLOMERULAR MESANGIAL CELLS; TUMOR-NECROSIS-FACTOR; ENDOTHELIAL-CELLS; MONONUCLEAR-CELLS; SURFACE-MEMBRANE; CROSS-REACT; T-CELLS; AUTOANTIBODIES; DSDNA;
Keywords:
monoclonal anti-dsDNA antibody; polymorphonuclear neutrophils; interleukin-8; apoptosis; activation-induced cell death;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Yu, CL Natl Yang Ming Univ, Sch Med, Inst Clin Med, Taipei 112, Taiwan Natl Yang Ming Univ Taipei Taiwan 112 lin Med, Taipei 112, Taiwan
Citazione:
S.C. Hsieh et al., "Monoclonal anti-double stranded DNA antibody is a leucocyte-binding protein to up-regulate interleukin-8 gene expression and elicit apoptosis of normal human polymorphonuclear neutrophils", RHEUMATOLOG, 40(8), 2001, pp. 851-858

Abstract

Objectives. To determine whether anti-double stranded DNA (anti-dsDNA) autoantibody could bind and affect the functions of normal human polymorphonuclear neutrophils (PMN). Methods. Normal human PMN were incubated with different concentrations of a monoclonal mouse anti-dsDNA antibody (12B3) or mouse isotype-matched IgG2a. The binding of anti-dsDNA and PMN was measured by flow cytometry and interleukin-8 (IL-8) gene expression in PMN was detected by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction(RT-PCR). PMN apoptosis was justified by morphological changes. The cognate antigen(s) of anti-dsDNA on the PMN surface was identified by membrane biotinylation, immunoprecipitation and Western blot. Results. The binding of PMN with anti-dsDNA was much higher than with non-specific mouse IgG2a (70.8 vs 2.0%). Anti-dsDNA at concentrations higher than 12.5 ng/ml significantly enhanced the production and mRNA expression of IL-8 by PMN. However, anti-dsDNA facilitated PMN apoptosis after 3 h incubation. Western blot analysis of biotinylated PMN cell lysates demonstrated that a 50-52 kDa membrane molecule is the cognate antigen of anti-dsDNA. Conclusions. Anti-dsDNA autoantibody up-regulates IL-8 gene expression andelicits activation-induced cell death (AICD) of human PMN via binding to a50-52 kDa membrane-expressed molecule.

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Documento generato il 03/04/20 alle ore 08:05:58