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Titolo:
Comparison of androgen receptor and oestrogen receptor beta immunoexpression in the testes of the common marmoset (Callithrix jacchus) from birth to adulthood: low androgen receptor immunoexpression in Sertoli cells during the neonatal increase in testosterone concentrations
Autore:
McKinnell, C; Saunders, PTK; Fraser, HM; Kelnar, CJH; Kivlin, C; Morris, KD; Sharpe, RM;
Indirizzi:
MRC, Ctr Reprod Biol, Human Reprod Sci Unit, Edinburgh EH3 9ET, Midlothian, Scotland MRC Edinburgh Midlothian Scotland EH3 9ET h EH3 9ET, Midlothian, Scotland Univ Edinburgh, Dept Reprod & Dev Sci, Sect Child Life & Hlth, Edinburgh EH9 1UW, Midlothian, Scotland Univ Edinburgh Edinburgh Midlothian Scotland EH9 1UW Midlothian, Scotland
Titolo Testata:
REPRODUCTION
fascicolo: 3, volume: 122, anno: 2001,
pagine: 419 - 429
SICI:
1470-1626(200109)122:3<419:COARAO>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
HORMONE ANTAGONIST; RAT TESTIS; GNRH ANTAGONIST; EXPRESSION; MONKEY; TISSUES; ALPHA; IMMUNOLOCALIZATION; SPERMATOGENESIS; LOCALIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: McKinnell, C MRC, Ctr Reprod Biol, Human Reprod Sci Unit, 37 Chalmers St, Edinburgh EH39ET, Midlothian, Scotland MRC 37 Chalmers St Edinburgh Midlothian Scotland EH3 9ET land
Citazione:
C. McKinnell et al., "Comparison of androgen receptor and oestrogen receptor beta immunoexpression in the testes of the common marmoset (Callithrix jacchus) from birth to adulthood: low androgen receptor immunoexpression in Sertoli cells during the neonatal increase in testosterone concentrations", REPRODUCT, 122(3), 2001, pp. 419-429

Abstract

The aims of this study were: (i) to investigate the cellular immunoexpression of androgen receptor and oestrogen receptor beta in the testes of the common marmoset (Callithrix jacchus) during neonatal life compared with their expression at later ages; (ii) to establish whether neonatal marmoset Sertoli cells are targets for androgens or oestrogens or both; and (iii) to investigate the relationship between neonatal plasma testosterone concentrations and androgen receptor immunoexpression by abolishing the neonatal testosterone surge with a potent GnRH antagonist. Androgen receptor and oestrogen receptor beta immunoexpression were evaluated in neonatal animals aged 1-4 days, 4 weeks and 6 weeks, and compared with immunoexpression in animals aged 18-22 weeks (early infancy), 35 weeks (late infancy), 58-62 weeks (late pubertal) and > 100 weeks (adult). Immunoexpression of androgen receptor in the reproductive tract was also evaluated at each age. Sertoli cell immunoexpression of androgen receptor was weak or absent in neonatal animals, but increased substantially in infant animals, reaching adult levels by the end of infancy. In contrast, immunoexpression of androgen receptor during the neonatal period was strong in testicular interstitial cells and very strong in epithelial cell nuclei throughout the reproductive tract, and did not change greatly with age in these cells or tissues. Similarly, immunoexpression of oestrogen receptor beta was prominent in many Sertoli cells and inthe germ cells of neonatal animals, and was relatively constant throughoutlife. Weak immunoexpression of androgen receptor in neonatal Sertoli cellswas associated with high plasma testosterone concentrations (2.7-5.5 ng ml(-1)), whereas strong Sertoli cell immunoexpression was associated with baseline (approximately 0.12 ng ml(-1)) testosterone concentrations in infant animals and with > 10 ng ml(-1) in late pubertal and adult animals. Immunoexpression of androgen receptor and oestrogen receptor beta was also evaluated in co-twin males aged 4 and 35 weeks, after treatment from birth to 4 weeks or from week 25 to week 35, respectively, with either vehicle or with GnRH antagonist at a dose known to suppress the neonatal testosterone surge completely. Only GnRH antagonist treatment during weeks 25-35 reduced androgen receptor immunoexpression, whereas immunoexpression of oestrogen receptor beta was unaffected by treatment during either period. On the basis of these findings it is suggested that: (i) neonatal marmoset Sertoli cells maybe targets primarily for oestrogens rather than androgens; (ii) androgen receptor expression in the testes of neonatal and infant marmosets is not regulated in a straightforward way by testosterone; and (iii) high neonatal concentrations of plasma testosterone are not absolutely necessary for expression of androgen receptor in marmoset testes at this time.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 12:51:21