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Titolo:
Alternative splicing isoforms of hippostasin (PRSS20/KLK11) in prostate cancer cell lines
Autore:
Nakamura, T; Mitsui, S; Okui, A; Kominami, K; Nomoto, T; Ukimura, O; Kawauchi, A; Miki, T; Yamaguchi, N;
Indirizzi:
Kyoto Prefectural Univ Med, Res Inst Geriatr, Dept Cell Biol, Kamigyo Ku, Kyoto 6028566, Japan Kyoto Prefectural Univ Med Kyoto Japan 6028566 Ku, Kyoto 6028566, Japan Kyoto Prefectural Univ Med, Dept Urol, Kamigyo Ku, Kyoto 6028566, Japan Kyoto Prefectural Univ Med Kyoto Japan 6028566 Ku, Kyoto 6028566, Japan Fuso Pharmaceut Co, Ctr Res & Dev, Osaka 5368523, Japan Fuso Pharmaceut Co Osaka Japan 5368523 r Res & Dev, Osaka 5368523, Japan
Titolo Testata:
PROSTATE
fascicolo: 1, volume: 49, anno: 2001,
pagine: 72 - 78
SICI:
0270-4137(20010915)49:1<72:ASIOH(>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
KALLIKREIN GENE FAMILY; SERINE-PROTEASE; MOLECULAR-CLONING; CDNA CLONING; EXPRESSION; BRAIN; TRANSCRIPT; NEUROPSIN; ANTIGEN; TLSP;
Keywords:
alternative splicing; hippostasin/PRSS20; prostate cancer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Yamaguchi, N Kyoto Prefectural Univ Med, Res Inst Geriatr, Dept Cell Biol,Kamigyo Ku, Kyoto 6028566, Japan Kyoto Prefectural Univ Med Kyoto Japan 6028566 28566, Japan
Citazione:
T. Nakamura et al., "Alternative splicing isoforms of hippostasin (PRSS20/KLK11) in prostate cancer cell lines", PROSTATE, 49(1), 2001, pp. 72-78

Abstract

Background. Hippostasin is a kallikrein-like protease (PRSS20/KLK11), which is expressed preferentially in the hippocampus and prostate. We have reported that alternative splicing variants of human hippostasin are regulated in a tissue-specific manner. Brain-type hippostasin consists of 250 amino acids including a typical signal sequence, and is expressed in the brain andprostate. The prostate-type hippostasin, which has 32 extra amino acids atthe N-terminal end, is expressed only in the prostate. Methods. We analyzed the expression and localization of hippostasin in normal prostate tissue, BPH tissue, and prostate cancer cell lines. We performed northern blotting, in situ hybridization, immunohistochemistry, and RT-PCR. Results. Hippostasin mRNA is expressed preferentially in the normal prostate and weakly in the testis. It was detected in prostate secretory epithelium. Hippostasin protein was localized in the prostate secretory epithelium,and western blotting showed that hippostasin was present in semen. All tested prostate cancer cell lines, including PSA-negative cell lines, expressed hippostasin. Interestingly, all the prostate cancer cell lines expressed only brain-type but not prostate-type hippostasin, while normal prostate and BPH expressed both types of hippostasin. Conclusions. Our results suggest the possibility that hippostasin may be auseful marker by which prostate cancer and BPH can be distinguished Prostate 49: 72-78, 2001. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 09:04:45