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Titolo:
A nuclear gene encoding mitochondrial triangle(1)-pyrroline-5-carboxylate dehydrogenase and its potential role in protection from proline toxicity
Autore:
Deuschle, K; Funck, D; Hellmann, H; Daschner, K; Binder, S; Frommer, WB;
Indirizzi:
ZMBP, D-72076 Tubingen, Germany ZMBP Tubingen Germany D-72076ZMBP, D-72076 Tubingen, Germany Univ Ulm, D-89069 Ulm, Germany Univ Ulm Ulm Germany D-89069Univ Ulm, D-89069 Ulm, Germany
Titolo Testata:
PLANT JOURNAL
fascicolo: 4, volume: 27, anno: 2001,
pagine: 345 - 355
SICI:
0960-7412(200108)27:4<345:ANGEMT>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRAMMED CELL-DEATH; ARABIDOPSIS-THALIANA; DELTA(1)-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE; SACCHAROMYCES-CEREVISIAE; STRESS; PLANTS; EXPRESSION; TRANSFORMATION; DEGRADATION; MUTANT;
Keywords:
Arabidopsis; cell death; pathogen response; proline metabolism; transcriptional regulation; yeast;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Frommer, WB ZMBP, Morgenstelle 1, D-72076 Tubingen, Germany ZMBP Morgenstelle 1 Tubingen Germany D-72076 ubingen, Germany
Citazione:
K. Deuschle et al., "A nuclear gene encoding mitochondrial triangle(1)-pyrroline-5-carboxylate dehydrogenase and its potential role in protection from proline toxicity", PLANT J, 27(4), 2001, pp. 345-355

Abstract

Delta (1)-pyrroline-5-carboxylate (P5C), an intermediate in biosynthesis and degradation of proline (Pro), is assumed to play a role in cell death inplants and animals. Toxicity of external Pro and P5C supply to Arabidopsissuggested that P5C dehydrogenase (P5CDH; EC 1.5.1.12) plays a crucial rolein this process by degrading the toxic Pro catabolism intermediate P5C. Also in a Delta put2 yeast mutant, lacking P5CDH, Pro led to growth inhibition and formation of reactive oxygen species (ROS). Complementation of the Delta put2 mutant allowed identification of the Arabidopsis P5CDH gene. AtP5CDH is a single-copy gene and the encoded protein was localized to the mitochondria. High homology of AtP5CDH to LuFIS1, an mRNA upregulated during susceptible pathogen attack in flax, suggested a role for P5CDH in inhibition of hypersensitive reactions. An Arabidopsis mutant (cpr5) displaying a constitutive pathogen response was found to be hypersensitive to external Pro. In agreement with a role in prevention of cell death, AtP5CDH was expressedat a basal level in all tissues analysed. The highest expression was foundin flowers that are known to contain the highest Pro levels under normal conditions. External supply of Pro induced AtP5CDH expression, but much moreslowly than Pro dehydrogenase (AtProDH) expression. Uncoupled induction ofthe AtProDH and AtP5CDH genes further supports the hypothesis that P5C levels have to be tightly controlled. These results indicate that, in additionto the well-studied functions of Pro, for example in osmoregulation, the Pro metabolism intermediate P5C also serves as a regulator of cellular stress responses.

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Documento generato il 29/11/20 alle ore 03:27:10