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Titolo:
Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in Arabidopsis
Autore:
Iuchi, S; Kobayashi, M; Taji, T; Naramoto, M; Seki, M; Kato, T; Tabata, S; Kakubari, Y; Yamaguchi-Shinozaki, K; Shinozaki, K;
Indirizzi:
RIKEN, Tsukuba Inst, Plant Mol Biol Lab, Tsukuba, Ibaraki 3050074, Japan RIKEN Tsukuba Ibaraki Japan 3050074 Lab, Tsukuba, Ibaraki 3050074, Japan Univ Shizouka, Fac Agr, Dept Forestry & Forest Resources, Shizuoka 4228529, Japan Univ Shizouka Shizuoka Japan 4228529 Resources, Shizuoka 4228529, Japan Kazusa DNA Res Inst, Chiba 2920812, Japan Kazusa DNA Res Inst Chiba Japan 2920812 A Res Inst, Chiba 2920812, Japan Minist Agr Forestry & Fisheries, Biol Resources Div, Japan Int Res Ctr AgrSci, Tsukuba, Ibaraki 3050851, Japan Minist Agr Forestry & Fisheries Tsukuba Ibaraki Japan 3050851 0851, Japan
Titolo Testata:
PLANT JOURNAL
fascicolo: 4, volume: 27, anno: 2001,
pagine: 325 - 333
SICI:
0960-7412(200108)27:4<325:RODTBG>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIGNAL-TRANSDUCTION; LOW-TEMPERATURE; TRANSCRIPTION FACTORS; FREEZING TOLERANCE; ALDEHYDE OXIDASE; WATER-DEFICIT; TOMATO MUTANT; EXPRESSION; THALIANA; STRESS;
Keywords:
abscisic acid (ABA); drought tolerance; transgenic plants; 9-cis-epoxycarotenoid dioxygenase (NCED);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Shinozaki, K RIKEN, Tsukuba Inst, Plant Mol Biol Lab, 3-1-1 Koyadai, Tsukuba, Ibaraki 3050074, Japan RIKEN 3-1-1 Koyadai Tsukuba Ibaraki Japan 3050074 0074, Japan
Citazione:
S. Iuchi et al., "Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in Arabidopsis", PLANT J, 27(4), 2001, pp. 325-333

Abstract

Abscisic acid (ABA), a plant hormone, is involved in responses to environmental stresses such as drought and high salinity, and is required for stress tolerance. ABA is synthesized de novo in response to dehydration. 9-cis-epoxycarotenoid dioxygenase (NCED) is thought to be a key enzyme in ABA biosynthesis. Here we demonstrate that the expression of an NCED gene of Arabidopsis, AtNCED3, is induced by drought stress and controls the level of endogenous ABA under drought-stressed conditions. Overexpression of AtNCED3 in transgenic Arabidopsis caused an increase in endogenous ABA level, and promoted transcription of drought- and ABA-inducible genes. Plants overexpressing AtNCED3 showed a reduction in transpiration rate from leaves and an improvement in drought tolerance. By contrast, antisense suppression and disruption of AtNCED3 gave a drought-sensitive phenotype. These results indicate that the expression of AtNCED3 plays a key role in ABA biosynthesis under drought-stressed conditions in Arabidopsis. We improved drought tolerance bygene manipulation of AtNCED3 causing the accumulation of endogenous ABA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 19:54:23