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Titolo:
Calcium, carbonic anhydrase and gastric acid secretion
Autore:
Puscas, I; Coltau, M; Baican, M; Domuta, G; Hecht, A;
Indirizzi:
Romanian Med Acad Sci, Ctr Res & Med Assistance, Salaj, Romania Romanian Med Acad Sci Salaj Romania es & Med Assistance, Salaj, Romania Univ Med Oradea, Oradea, Romania Univ Med Oradea Oradea RomaniaUniv Med Oradea, Oradea, Romania Clin Wilkenberg, Wilkenberg, Germany Clin Wilkenberg Wilkenberg GermanyClin Wilkenberg, Wilkenberg, Germany Med Care Unit, New York, NY USA Med Care Unit New York NY USAMed Care Unit, New York, NY USA
Titolo Testata:
PHYSIOLOGICAL RESEARCH
fascicolo: 4, volume: 50, anno: 2001,
pagine: 359 - 364
SICI:
0862-8408(2001)50:4<359:CCAAGA>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENZYME;
Keywords:
calcium; disodium EDTA; carbonic anhydrase; gastric acid secretion;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Puscas, I Romanian Med Acad Sci, Ctr Res & Med Assistance, 4775 Simleu Silvaniei,37 Dunarii St, Salaj, Romania Romanian Med Acad Sci 4775 Simleu Silvaniei,37 Dunarii St Salaj Romania
Citazione:
I. Puscas et al., "Calcium, carbonic anhydrase and gastric acid secretion", PHYSL RES, 50(4), 2001, pp. 359-364

Abstract

Previous data concerning the action of calcium (Ca) on gastric acid secretion (GAS) indicated that calcium ions increase GAS elicited by gastrin released through a vagal mechanism, and also by a direct effect on parietal cells. Our research showed that the stimulating effect of calcium on gastric acid secretion can be antagonized by verapamil administration, which reducesgastric acid secretion. In the present study we followed the effect induced by administration of calcium and Ca-chelating agents (disodium EDTA) on gastric acid secretion and on carbonic anhydrase (CA) activity. We selected two groups of healthy volunteers: Group I (n=21) received a single i.v. dose of CaCl2 (15 mg/kg b.w.), whereas Group II (n=22) received a single i.v. dose of disodium EDTA (5 mg/kg b.w.). We determined blood calcium before and after treatment, gastric acid secretion at 2 hours, erythrocyte CA II activity, and CA IV activity in membrane parietal cells, which were isolated from gastric mucosa obtained by endoscopic biopsy. Assessment of carbonic anhydrase activity was achieved by the stopped-flow method. In Group I calcium administration increased blood calcium, HCl output, CA II and CA IV activity as compared to initial values. In Group II, disodium EDTA reduced bloodcalcium, HCl output, CA II and CA IV activity as compared to initial values. The results demonstrated that increased blood calcium and GAS values after calcium administration correlated with the increase of erythrocyte CA IIand parietal cell CA IV activity, while disodium EDTA induced a reversed process. Our results also show that cytosolic CA II and membrane CA IV values are sensitive to calcium changes and they directly depend on these levels. Our data suggest that intra- and extracellular pH changes induced by carbonic anhydrase might account for the modulation of the physiological and pathological secretory processes in the organism.

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Documento generato il 29/03/20 alle ore 01:33:06