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Titolo:
PCR-based detection of sterol demethylation inhibitor-resistant strains ofPenicillium digitatum
Autore:
Hamamoto, H; Hasegawa, K; Nakaune, R; Lee, YJ; Akutsu, K; Hibi, T;
Indirizzi:
Univ Tokyo, Dept Agr & Environm Biol, Plant Pathol Lab, Bunkyo Ku, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 thol Lab, Bunkyo Ku, Tokyo 1138657, Japan Natl Inst Fruit Tree Sci, Akitsu, Hiroshima 7292494, Japan Natl Inst FruitTree Sci Akitsu Hiroshima Japan 7292494 ma 7292494, Japan Ibaraki Univ, Fac Agr, Ami, Ibaraki 3000393, Japan Ibaraki Univ Ami Ibaraki Japan 3000393 c Agr, Ami, Ibaraki 3000393, Japan
Titolo Testata:
PEST MANAGEMENT SCIENCE
fascicolo: 9, volume: 57, anno: 2001,
pagine: 839 - 843
SICI:
1526-498X(200109)57:9<839:PDOSDI>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
CITRUS-FRUIT; IMAZALIL; ITALICUM; BIOSYNTHESIS; SENSITIVITY; FUNGICIDES; CALIFORNIA; BIOTYPES;
Keywords:
spore PCR; demethylation inhibitor fungicide resistance; Penicillium digitatum;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Hamamoto, H Univ Tokyo, Dept Agr & Environm Biol, Plant Pathol Lab, BunkyoKu, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 nkyo Ku, Tokyo 1138657, Japan
Citazione:
H. Hamamoto et al., "PCR-based detection of sterol demethylation inhibitor-resistant strains ofPenicillium digitatum", PEST MAN SC, 57(9), 2001, pp. 839-843

Abstract

A simple method for detecting sterol demethylation inhibitor (DMI)-resistant strains of the citrus green mould pathogen, Penicillium digitatum, has been developed. The method involves detection of a tandem repeat of a transcriptional enhancer in the promoter region of PdCYP51, which encodes the target enzyme of DMIs, by PCR, using conidia as template. The presence of the tandem repeat leads to overexpression of this gene and confers DMI resistance to the fungus. We examined the relationship between the presence of the tandem repeat and DMI resistance in 39 strains of P digitatum. The results suggested that the DMI resistance mechanism based on the tandem repeat is common in this fungus. Using this method, the presence of DMI resistance of this fungus can be detected within 5-6 h. (C) 2001 Society of Chemical Industry.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/05/20 alle ore 15:19:03