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Titolo:
Substance P, calcitonin gene related peptide and PGE(2) co-released from the mouse colon: a new model to study nociceptive and inflammatory responsesin viscera, in vitro
Autore:
Roza, C; Reeh, PW;
Indirizzi:
Univ Erlangen Nurnberg, Dept Physiol & Expt Pathophysiol, D-91054 Erlangen, Germany Univ Erlangen Nurnberg Erlangen Germany D-91054 -91054 Erlangen, Germany
Titolo Testata:
PAIN
fascicolo: 3, volume: 93, anno: 2001,
pagine: 213 - 219
SICI:
0304-3959(200109)93:3<213:SPCGRP>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
SENSITIVE SENSORY NERVES; AFFERENT-FIBERS; PROSTAGLANDIN E-2; IN-VITRO; THERAPEUTIC IMPLICATIONS; COLORECTAL DISTENSION; MOLECULAR MECHANISMS; CAPSAICIN RECEPTOR; RAT SKIN; PAIN;
Keywords:
capsaicin; noxious heat; protons; distension; visceral pain;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Reeh, PW Univ Erlangen Nurnberg, Dept Physiol & Expt Pathophysiol, Univ Str 17, D-91054 Erlangen, Germany Univ Erlangen Nurnberg Univ Str 17 Erlangen Germany D-91054 many
Citazione:
C. Roza e P.W. Reeh, "Substance P, calcitonin gene related peptide and PGE(2) co-released from the mouse colon: a new model to study nociceptive and inflammatory responsesin viscera, in vitro", PAIN, 93(3), 2001, pp. 213-219

Abstract

Visceral inflammation is thought to play an important role in the sensitization of low and high threshold mechanosensory and polymodal afferents and to recruit silent nociceptors. Yet, little is known about the potential role of the mediators involved in nociceptor sensitization to mechanical stimulation as compared to heat sensitization in the skin. In the present study we developed a new isolated preparation of the mouse colon which allowed toapply controlled mechanical distensions. Excised segments of colon from CDmice were immersed in synthetic interstitial fluid (SIF) exposing the serosal surface during 5 min to different types of noxious stimuli; the increase in neuropeptide and PGE(2) release were analyzed (by EIA technique). Capsaicin, heat and pH 5.2 were able to induce significant increases in calcitonin gene related peptide (CORP) release (14.6-, 5.1-, and 2.3-fold over baseline), however, only capsaicin induced a significant increase in substanceP (SP) levels (1.8-fold over baseline). When pH 3.4 was used, a massive liberation of both CORP and SP was obtained (14- and 15-fold from baseline) which was Ca2+-independent and not recovering, suggesting unphysiological. release. Mechanical distensions in the noxious range (45, 60 and 90 mmHg) evoked a long-linear graded release of CGRP (1.3-, 1.6- and 2.6-fold over baseline) and of PGE(2) (1.9- 3.8-, 12.3-fold over baseline). Only the 90 mmHgdistension evoked a significant increase of SP (1.9-fold over baseline). We conclude that the mouse colon preparation is a suitable model to study inflammatory and nociceptive mechanisms in viscera. Furthermore, a potentially important and yet unexplored role of PGE(2) in noxious visceral distension has been revealed. (C) 2001 International Association for the Study of Pain. Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 18:27:17