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Titolo:
COCH5B2 is a target antigen of anti-inner ear antibodies in autoimmune inner ear diseases
Autore:
Boulassel, MR; Tomasi, JP; Deggouj, N; Gersdorff, M;
Indirizzi:
Univ Catholique Louvain, Sch Med, Dept Microbiol, Lab Autoimmun, B-1200 Brussels, Belgium Univ Catholique Louvain Brussels Belgium B-1200 B-1200 Brussels, Belgium Univ Catholique Louvain, Sch Med, Dept Otorhinolaryngol, B-1200 Brussels, Belgium Univ Catholique Louvain Brussels Belgium B-1200 B-1200 Brussels, Belgium
Titolo Testata:
OTOLOGY & NEUROTOLOGY
fascicolo: 5, volume: 22, anno: 2001,
pagine: 614 - 618
SICI:
1531-7129(200109)22:5<614:CIATAO>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
SENSORINEURAL HEARING-LOSS; MENIERES-DISEASE; EXTRACELLULAR-MATRIX; AUTOANTIBODIES; PROTEINS; GENE; MUTATION; DEAFNESS; DOMAINS;
Keywords:
inner ear diseases; antibodies; Edman degradation; COCH5B2 protein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Boulassel, MR Univ Catholique Louvain, Sch Med, Dept Microbiol, Lab Autoimmun, UCl 5490,Ave Hippocrate 54, B-1200 Brussels, Belgium Univ Catholique Louvain UCl 5490,Ave Hippocrate 54 Brussels Belgium B-1200
Citazione:
M.R. Boulassel et al., "COCH5B2 is a target antigen of anti-inner ear antibodies in autoimmune inner ear diseases", OTOL NEURO, 22(5), 2001, pp. 614-618

Abstract

Objective: This study was designed to identify the 58-kDa inner ear protein against which the sera of some patients with idiopathic, progressive sensorineural hearing loss or Meniere's disease strongly react. Background: We and other groups have previously demonstrated that a 58-kDaprotein extracted from guinea pig or bovine inner ear tissue is a target of antibodies in serum samples from some patients with autoimmune inner ear diseases. Methods: After separation of inner ear proteins by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis, the bands corresponding to 58 kDa were localized and excised from the gel. The concentrated protein was then digested with trypsin, and the peptide fragments were separated by high-pressure liquid chromatography. Three fractions were subjected to amino acid sequencing by the classic Edman degradation. Results: The sequence of a stretch of 14 amino acids of the first fragmentwas identical to that of amino acids 526 to 539 of the COCH5B2 protein. The sequences of 11 and 10 amino acids of the second and third fragments, respectively, also were identical to residues 417 to 427 and 396 to 405 of theCOCH5B2 protein. These data, together with two-dimensional gel electrophoresis followed by Western blot experiments, confirmed that the 58-kDa inner ear protein is the COCH5B2 protein. Discussion: These findings indicate that the 58-kDa target protein of antibodies in serum samples of patients with autoimmune inner ear diseases is the COCH5B2 protein, a molecule that is highly and specifically expressed inthe cochlea and vestibule.

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Documento generato il 29/02/20 alle ore 02:41:37