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Titolo:
Temporal and spatial patterns of DNA fragmentation following focally or systemically-evoked status epilepticus in rats
Autore:
Kondratyev, A; Gale, K;
Indirizzi:
Georgetown Univ, Med Ctr, Dept Pharmacol, Washington, DC 20007 USA Georgetown Univ Washington DC USA 20007 armacol, Washington, DC 20007 USA
Titolo Testata:
NEUROSCIENCE LETTERS
fascicolo: 1, volume: 310, anno: 2001,
pagine: 13 - 16
SICI:
0304-3940(20010907)310:1<13:TASPOD>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACID-INDUCED SEIZURES; KAINIC ACID; NEURONAL APOPTOSIS; CELL-DEATH; BRAIN; ACTIVATION; KAINATE; INJURY;
Keywords:
status epilepticus; seizure; kainic acid; DNA fragmentation; apoptosis; hippocampus; rhinal cortex; rat; silver staining;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Kondratyev, A Georgetown Univ, Med Ctr, Dept Pharmacol, Res Bldg,Room W217,3970 Reservoir Rd NW, Washington, DC 20007 USA Georgetown Univ Res Bldg,Room W217,3970 Reservoir Rd NW Washington DC USA 20007
Citazione:
A. Kondratyev e K. Gale, "Temporal and spatial patterns of DNA fragmentation following focally or systemically-evoked status epilepticus in rats", NEUROSCI L, 310(1), 2001, pp. 13-16

Abstract

Status epilepticus (SE) triggers neuronal degeneration comprised of both necrotic and apoptotic components. Here we determined whether internucleosomal DNA fragmentation reflects the severity of SE-induced neuronal damage. We utilized both a systemic (kainic acid) and a focally-induced model of SE in rats. DNA fragmentation was analyzed in rhinal cortex and hippocampus atvarious time points following SE episodes of varying durations (30-120 min). Radioactively labeled DNA fragments were analyzed by agarose gel electrophoresis and quantified by liquid scintillation counting. The spatial and temporal characteristics of the SE-evoked DNA fragmentation indicated that this marker of apoptosis appears as early as 8 h after SE and reaches peak expression at 48 h. This method permitted us to quantitatively monitor the evolution of the apoptotic component of cell death over the acute post-injury period (8-72 h). Moreover, in both models of SE, the DNA fragmentation varied directly as a linear function of the duration of SE between 30 and 120min suggesting that this marker should be highly responsive to neuroprotective intervention. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 16:26:35