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Titolo:
IMMUNIZATION WITH A MULTIPLE ANTIGEN PEPTIDE-CONTAINING DEFINED B-CELL AND T-CELL EPITOPES - PRODUCTION OF BACTERICIDAL ANTIBODIES AGAINST GROUP-B NEISSERIA-MENINGITIDIS
Autore:
CHRISTODOULIDES M; HECKELS JE;
Indirizzi:
UNIV SOUTHAMPTON,SOUTHAMPTON GEN HOSP,TREMONA RD SOUTHAMPTON SO18 6YDHANTS ENGLAND
Titolo Testata:
Microbiology
, volume: 140, anno: 1994,
parte:, 11
pagine: 2951 - 2960
SICI:
1350-0872(1994)140:<2951:IWAMAP>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
OUTER-MEMBRANE PROTEIN; SYNTHETIC PEPTIDES; MENINGOCOCCAL VACCINE; CIRCUMSPOROZOITE PROTEIN; DESIGN; IMMUNOGENICITY; DISEASE; SYSTEM; GONORRHOEAE; PROTECTION;
Keywords:
NEISSERIA MENINGITIDIS; MULTIPLE ANTIGEN PEPTIDE; OUTER MEMBRANE PROTEIN; T HELPER-CELL EPITOPE; B-CELL EPITOPE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
M. Christodoulides e J.E. Heckels, "IMMUNIZATION WITH A MULTIPLE ANTIGEN PEPTIDE-CONTAINING DEFINED B-CELL AND T-CELL EPITOPES - PRODUCTION OF BACTERICIDAL ANTIBODIES AGAINST GROUP-B NEISSERIA-MENINGITIDIS", Microbiology, 140, 1994, pp. 2951-2960

Abstract

Previous analysis of the class 1 outer-membrane (OM) protein of Neisseria meningitidis has identified discrete epitopes to be potential targets for immune attack. The conformation of these epitopes is important for inducing antibodies which can react with the native protein and promote complement-mediated lysis of the meningococcus. The multiple antigen peptide (MAP) system, which consists of an oligomeric branchinglysine core to which are attached dendritic arms of defined peptide antigens. confers some conformational stability and also allows for thepreparation of immunogens containing both B-cell and T helper (fh)-cell epitopes. In this study, MAPs were synthesized to contain (i) the subtype P1.16b meningococcal class 1 protein B-cell epitope (B-MAP). and (ii) the P1.16b epitope in tandem with a defined Th-cell epitope, chosen from tetanus toxin (BT-MAP). The B-MAP was nonimmunogenic in animals. In contrast, incorporation of the Th-cell epitope into PT-MAP induced a strong humoral response towards the class 1 protein B-cell epitope. Antisera from immunized mice and rabbits reacted in ELISA with synthetic peptides containing the B-cell epitope. and also cross-reactedwith meningococcal OMs from strains of subtype P1.16b and P1.16a. Murine and rabbit antisera showed similar reactivity and epitope specificity, but did not react with denatured class 1 protein in Western blotting, indicating the predominance of antibodies directed towards conformational epitopes. The antisera from rabbits immunized with BT-MAP promoted complement-mediated bactericidal killing not only of the homologous meningococcal subtype P1.16b strain but also of subtype P1.16a.

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Documento generato il 02/12/20 alle ore 08:02:52