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Titolo:
Production of monokines in patients under polysulphone haemodiafiltration is influenced by the ultrafiltration flow rate
Autore:
Muller-Steinhardt, M; Kock, N; Hartell, C; Kirchner, H; Steinhoff, J;
Indirizzi:
Univ Lubeck, Inst Immunol & Transfus Med, Sch Med, D-23538 Lubeck, GermanyUniv Lubeck Lubeck Germany D-23538 Med, Sch Med, D-23538 Lubeck, Germany Univ Lubeck, Dept Nephrol, Sch Med, D-23538 Lubeck, Germany Univ Lubeck Lubeck Germany D-23538 rol, Sch Med, D-23538 Lubeck, Germany
Titolo Testata:
NEPHROLOGY DIALYSIS TRANSPLANTATION
fascicolo: 9, volume: 16, anno: 2001,
pagine: 1830 - 1837
SICI:
0931-0509(200109)16:9<1830:POMIPU>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
NECROSIS-FACTOR-ALPHA; HEMODIALYSIS-PATIENTS; DIALYSIS PATIENTS; BLOOD MONOCYTES; MORTALITY; BETA-2-MICROGLOBULIN; INTERLEUKIN-6; MACROPHAGES; MEMBRANES; RESPONSES;
Keywords:
haemodiafiltration; immunocompetence; monokines; ultrafiltration flow rate;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Muller-Steinhardt, M Univ Lubeck, Inst Immunol & Transfus Med, Sch Med, Ratzeburger Allee 160, D-23538 Lubeck, Germany Univ Lubeck Ratzeburger Allee 160 Lubeck Germany D-23538
Citazione:
M. Muller-Steinhardt et al., "Production of monokines in patients under polysulphone haemodiafiltration is influenced by the ultrafiltration flow rate", NEPH DIAL T, 16(9), 2001, pp. 1830-1837

Abstract

Background. Chronic haemodialysis patients show various clinical signs of immunodeficiency and there is growing evidence that a dysregulated monocytecytokine, production is heavily involved in this deficiency. The production of monokines in vitro has been proposed to correlate closely with the in vivo immune status and to be of high clinical relevance in cuprophane haemodialysis. Even though it is well known that the biocompatibility of dialyser membranes has a significant impact on immune functions, little is known about the influence of the ultrafiltration flow rate (UFR). The aim of this study was to investigate the potential long-term effects of UFR on the production of interleukin-10 (IL-10), interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6) in an intra-individual study design. Methods. In I I patients previously treated with polysulphone haemodiafiltration, UFR was reduced from 40-46 ml/min to 24-28 ml/min, then to 7-10 ml/min before it was reinstated at 40-46 ml/min for periods of 4 weeks each. Monokine secretion into culture supernatants. and mRNA expression (assessed using a novel Taqman PCR technique), were determined in a whole blood assayafter lipopolysaccharide stimulation. Results. Reduction of UFR led to a significant increase in IL-10 secretionand mRNA expression (P=0.012, P=0.001). Conversely, a substantial (but notcomplete) decrease was observed when UFR returned to initial levels. In contrast, supernatant concentrations of IL-1 beta (P=0.04) and IL-6 (P=0.003), and mRNA expression of both monokines (P <0.001, P <0.001) decreased significantly when UFR was reduced. Calculation of the IL-1 beta /IL-10 ratio also revealed a decrease when UFR was reduced, with an increase again being observed when the initial degree of UFR was reinstated (P <0.001). Conclusions. These results indicate a significant impact of UFR on the production of monokines at both the transcriptional and the protein level. We suggest that middle molecule removal has to be considered as a possible pathophysiological mechanism to explain our findings. Since monokine production in vitro was shown to be closely correlated with the in vivo immune status in patients on cuprophane haemodialysis, further investigations are necessary to clarify the impact of UFR on the immunocompetence of patients underpolysulphone haemodiafiltration.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 06:55:48