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Titolo:
Molecular cloning of kpcA gene encoding a Kex2p-like endoprotease from Aspergillus nidulans
Autore:
Kwon, BK; Han, KH; Han, KY; Ju, SM; Hwang, SG; Jeon, BH; Han, DM; Kim, WS;
Indirizzi:
Wonkwang Univ, Coll Nat Sci, Div Life Sci, Iksan 570749, South Korea Wonkwang Univ Iksan South Korea 570749 fe Sci, Iksan 570749, South Korea Wonkwang Univ, Coll Oriental Med, Dept Pathol, Iksan 570749, South Korea Wonkwang Univ Iksan South Korea 570749 Pathol, Iksan 570749, South Korea
Titolo Testata:
MOLECULES AND CELLS
fascicolo: 1, volume: 12, anno: 2001,
pagine: 142 - 147
SICI:
1016-8478(20010831)12:1<142:MCOKGE>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROCESSING ENDOPROTEASE; FUSION PROTEIN; FURIN; PROHORMONE; SECRETION; CLEAVAGE; PROPEPTIDE; NIGER; INTERLEUKIN-6; CONVERTASES;
Keywords:
KexB; kexin; proprotein convertase; proprotein processing; subtilisin serine protease;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, WS Wonkwang Univ, Coll Nat Sci, Div Life Sci, Iksan 570749, South Korea Wonkwang Univ Iksan South Korea 570749 Iksan 570749, South Korea
Citazione:
B.K. Kwon et al., "Molecular cloning of kpcA gene encoding a Kex2p-like endoprotease from Aspergillus nidulans", MOL CELLS, 12(1), 2001, pp. 142-147

Abstract

We cloned and sequenced a gene, kpcA ((K) under bar ex2p-like (p) under bar roprotein (c) under bar onvertase A), from a genomic library of Aspergillus nidulans. The kpcA gene encodes an 820-residue protein, named KpcA, which contains a putative subtilisin-like catalytic domain (residues 136-466) homologous to that of the subtilisin serine protease family. KpcA shows 56, 73, and 47% amino acid identities with Saccharomyces cerevisiae Kex2p, Aspergillus niger KexB, and mouse furin within the subtilisin-like catalytic domain, respectively. The sequences around the proposed active site Asp, His,and Ser residues of KpcA are similar to those of other Kex2p family members. The KpcA mRNA transcript with an expected size of similar to2.8 kb was detected in A. nidulans. The substrate specificity of KpcA, expressed in CHOcells, is similar to that of A. niger KexB and yeast Kex2p. We conclude that KpcA is a resident Kex2p-like proprotein that processes endoprotease in A. nidulans.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 15:01:01