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Titolo:
Immune induction and modulation in mice following immunization with DNA encoding F protein of respiratory syncytial virus
Autore:
Park, EK; Soh, BY; Jang, YS; Park, JH; Chung, GH;
Indirizzi:
Chonbuk Natl Univ, Coll Nat Sci, Fac Biol Sci, Chonju 561756, South Korea Chonbuk Natl Univ Chonju South Korea 561756 , Chonju 561756, South Korea Changwon Univ, Dept Biol, Chang Won 641773, South Korea Changwon Univ Chang Won South Korea 641773 Chang Won 641773, South Korea
Titolo Testata:
MOLECULES AND CELLS
fascicolo: 1, volume: 12, anno: 2001,
pagine: 50 - 56
SICI:
1016-8478(20010831)12:1<50:IIAMIM>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMID DNA; VACCINE CANDIDATE; MESSENGER-RNA; RESPONSES; GLYCOPROTEIN; PROTECTION; SEQUENCE; GENES; TH1; RSV;
Keywords:
cytokine; DNA immunization; F protein; respiratory syncytial virus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Chung, GH Chonbuk Natl Univ, Coll Nat Sci, Fac Biol Sci, Chonju 561756, South Korea Chonbuk Natl Univ Chonju South Korea 561756 61756, South Korea
Citazione:
E.K. Park et al., "Immune induction and modulation in mice following immunization with DNA encoding F protein of respiratory syncytial virus", MOL CELLS, 12(1), 2001, pp. 50-56

Abstract

Respiratory syncytial virus (RSV) is one of the principal agents of bronchiolitis and pneumonia in young children. Thus, there is a strong need to make a safe and effective vaccine against the RSV infection. DNA immunizationis very effective at inducing both cellular and humoral immune responses. In this study, we inserted the RSV-F gene into expression vectors, pcDNA3.1and pQE. These constructs were transformed into C2C12 and E.coli M15 cells, respectively. The expression of the RSV-F protein was confirmed by SDS-PAGE, followed by Western blot analyses. The immunization of pcDNA3.1-RSV-F elicited both anti-RSV-F titer in mouse sera and CTL activities with mouse splenocytes. Especially, the co-administration of IL-4, or the GM-CSF gene with the RSV-F gene construct, enhanced the production of anti-RSV-F Ab. However, this enhancement disappeared by the simultaneous injection of the Th1and Th2 type cytokine genes. The CTL activities were affected by the co-delivery of the IFN-gamma gene, but not by Th2-type cytokines.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 13:07:20