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Titolo:
Induction of apoptosis in p53-deficient human hepatoma cell line by wild-type p53 gene transduction: Inhibition by antioxidant
Autore:
Lee, KH; Kim, KC; Jung, YJ; Ham, YH; Jang, JJ; Kwon, H; Sung, YC; Kim, SH; Han, SK; Kim, CM;
Indirizzi:
Korea Canc Ctr Hosp, Mol Oncol Lab, Seoul 139240, South Korea Korea Canc Ctr Hosp Seoul South Korea 139240 , Seoul 139240, South Korea Seoul Natl Univ Hosp, Dept Internal Med, Seoul 110799, South Korea Seoul Natl Univ Hosp Seoul South Korea 110799 Seoul 110799, South Korea Pohang Univ Sci & Technol, Dept Life Sci & Technol, Pohang 790849, South Korea Pohang Univ Sci & Technol Pohang South Korea 790849 790849, South Korea Kyung Hee Univ, Inst Basic Res, Dept Biol, Seoul 130701, South Korea KyungHee Univ Seoul South Korea 130701 Biol, Seoul 130701, South Korea
Titolo Testata:
MOLECULES AND CELLS
fascicolo: 1, volume: 12, anno: 2001,
pagine: 17 - 24
SICI:
1016-8478(20010831)12:1<17:IOAIPH>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN HEPATOCELLULAR CARCINOMAS; LUNG-CANCER CELLS; GROWTH SUPPRESSION; ADENOVIRUS; EXPRESSION; PROTEIN; WAF1/CIP1; RADICALS; THERAPY; ARREST;
Keywords:
p21(WAF1-CIP1); p53; antioxidant; apoptosis; hepatocellular carcinoma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, CM Natl Canc Ctr, Koyang 411351, South Korea Natl Canc Ctr Koyang South Korea 411351 yang 411351, South Korea
Citazione:
K.H. Lee et al., "Induction of apoptosis in p53-deficient human hepatoma cell line by wild-type p53 gene transduction: Inhibition by antioxidant", MOL CELLS, 12(1), 2001, pp. 17-24

Abstract

We investigated the role of wild-type (wt)-p53 as an inducer of apoptotic cell death in human hepatoma cell lines. Following the retrovirus-mediated transduction of the wt-p53 gene, Hep3B cells lacking the endogenous p53 expression began to die through apoptosis in 4 h. They showed a maximal apoptotic death at 12 h, whereas HepG2 cells expressing endogenous p53 did not. However, the transduction of the wt-p53 gene elicited growth suppression of both Hep3B and HepG2 cells. P21(WAF1/CIP1), a p53-inducible cell cycle inhibitor, was induced, not only in Hep3B cells undergoing apoptosis, but also in HepG2 cells. The kinetics of the p21(WAF1/CIP1) induction, DNA fragmentation, and growth suppression of the Hep3B cells showed that DNA fragmentation and growth suppression progressed rapidly following p21(WAF1/CIP1) accumulation. N-acetylcysteine or glutathione, potent antioxidants, strongly inhibited the DNA fragmentation, but did not reduce the elevated level of p21(WAF1/CIP1). These findings suggested that p21(WAF1/CIP1) was not a criticalmediator for the execution of p53-mediated apoptosis, although it contributed to the growth inhibition of cells undergoing apoptosis. Furthermore, p53-mediated apoptosis could be repressed by antioxidants.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:32:30