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Titolo:
Expression of the alpha(1F) calcium channel subunit by photoreceptors in the rat retina
Autore:
Morgans, CW; Gaughwin, P; Maleszka, R;
Indirizzi:
Australian Natl Univ, John Curtin Sch Med Res, Div Neurosci, Synapt Biochem Grp, Canberra, ACT 0200, Australia Australian Natl Univ Canberra ACT Australia 0200 rra, ACT 0200, Australia Australian Natl Univ, Res Sch Biol Sci, Canberra, ACT 0200, Australia Australian Natl Univ Canberra ACT Australia 0200 rra, ACT 0200, Australia
Titolo Testata:
MOLECULAR VISION
fascicolo: 28, volume: 7, anno: 2001,
pagine: 202 - 209
SICI:
1090-0535(20010822)7:28<202:EOTACC>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
STATIONARY NIGHT BLINDNESS; 2 SIZE FORMS; BIPOLAR CELLS; CONE PHOTORECEPTORS; ALPHA-1 SUBUNIT; CURRENTS; RELEASE; GENE; LOCALIZATION; RIBBON;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Morgans, CW Australian Natl Univ, John Curtin Sch Med Res, Div Neurosci, Synapt Biochem Grp, Canberra, ACT 0200, Australia Australian Natl Univ Canberra ACT Australia 0200 0, Australia
Citazione:
C.W. Morgans et al., "Expression of the alpha(1F) calcium channel subunit by photoreceptors in the rat retina", MOL VIS, 7(28), 2001, pp. 202-209

Abstract

Purpose: The CACNA1F gene encodes a voltage-gated calcium channel alpha (1) subunit, alpha (1F), which is expressed in the human retina. Mutations inthis gene cause incomplete X-linked congenital stationary night blindness (CSNB2). The aim of this study was to obtain the sequence of the rat alpha (1F) cDNA and localize the encoded polypeptide in the rat retina. Methods: The full-length rat alpha (1F) sequence was compiled from sequencing of overlapping alpha (1F) PCR fragments amplified from rat retinal cDNA. Antiserum was raised against a human alpha (1F) peptide. It was found that the human alpha (1F) peptide used to generate the antiserum was conservedat only 11 out of 19 residues in the cloned rat sequence. Therefore, antibodies were affinity purified against either the human alpha (1F) peptide orthe equivalent rat peptide and used for immunofluorescent staining of rat retina sections. Results: The rat alpha (1F) amino acid sequence was found to be 91% and 95% identical to the human and mouse alpha (1F) sequences, respectively. Antibodies affinity purified against the human alpha (1F) peptide stained both the outer nuclear layer (ONL) and outer plexiform layer of rat retina sections. In contrast, staining with antibodies affinity purified against the corresponding rat alpha (1F) peptide was restricted to the ONL. Conclusions: The rat alpha (1F) amino acid sequence is highly homologous to the human and mouse sequences. The immunohistochemical results indicate the existence of distinct alpha (1F) isoforms or alpha (1F)-like channels, which are differentially distributed in the cell bodies and synaptic terminals of photoreceptors in the rat retina.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 00:11:20