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Titolo:
Transcriptional regulation of human placental leucine aminopeptidase/oxytocinase gene
Autore:
Ito, T; Nomura, S; Okada, M; Katsumata, Y; Iwase, A; Kikkawa, F; Tsujimoto, M; Mizutani, S;
Indirizzi:
Nagoya Univ, Sch Med, Dept Obstet & Gynecol, Nagoya, Aichi 4668550, Japan Nagoya Univ Nagoya Aichi Japan 4668550 ecol, Nagoya, Aichi 4668550, Japan RIKEN, Inst Phys & Chem Res, Lab Cellular Biochem, Wako, Saitama 3510148, Japan RIKEN Wako Saitama Japan 3510148 ar Biochem, Wako, Saitama 3510148, Japan
Titolo Testata:
MOLECULAR HUMAN REPRODUCTION
fascicolo: 9, volume: 7, anno: 2001,
pagine: 887 - 894
SICI:
1360-9947(200109)7:9<887:TROHPL>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN CHORIONIC-GONADOTROPIN; NORMAL-PREGNANCY; RECEPTOR GENE; STRUCTURAL ORGANIZATION; MEMBRANE AMINOPEPTIDASE; GLUT4 VESICLES; MAJOR PROTEIN; SUBUNIT GENE; PROMOTER; EXPRESSION;
Keywords:
aminopeptidase; AP-2; oxytocinase; placenta; promoter;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Nomura, S Nagoya Univ, Sch Med, Dept Obstet & Gynecol, Nagoya, Aichi 4668550, Japan Nagoya Univ Nagoya Aichi Japan 4668550 ya, Aichi 4668550, Japan
Citazione:
T. Ito et al., "Transcriptional regulation of human placental leucine aminopeptidase/oxytocinase gene", MOL HUM REP, 7(9), 2001, pp. 887-894

Abstract

Human placental leucine aminopeptidase (P-LAP) plays a major role in the clearance of oxytocin, which is a key hormone in regulating labour pain. To explore the transcriptional regulation of P-LAP gene expression in placenta, we performed systematic studies using human choriocarcinoma cells, BeWo and JEG-3, as a model of placental trophoblastic cells. Transient transfection and luciferase assays using various 5 ' -deleted P-LAP-luciferase constructs showed that the region from -297 to +49 of the transcription start site was responsible for promoter activity in these cells. Footprinting analysis with nuclear extracts from both cell lines demonstrated at least four sites for nucleoprotein interactions in this region (FP1 to FP4). Site-directed deletion of FP1-4 in luciferase assays indicated the significance of theFP3 region (-214 to -183) for high promoter activity in the cells. Electrophoretic mobility shift assays to identify the proteins interacting with DNA at FP3 revealed three retarded bands, one of which was generated by activator protein-2 (AP-2) binding. Our findings suggest that AP-2 may be one ofthe important factors regulating P-LAP gene expression in human placenta.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 18:42:37