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Titolo:
Germ cell specific expression of c-kit in the human fetal gonad
Autore:
Robinson, LLL; Gaskell, TL; Saunders, PTK; Anderson, RA;
Indirizzi:
MRC, Human Reprod Sci Unit, Edinburgh EH3 9ET, Midlothian, Scotland MRC Edinburgh Midlothian Scotland EH3 9ET h EH3 9ET, Midlothian, Scotland
Titolo Testata:
MOLECULAR HUMAN REPRODUCTION
fascicolo: 9, volume: 7, anno: 2001,
pagine: 845 - 852
SICI:
1360-9947(200109)7:9<845:GCSEOC>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
LASER CAPTURE MICRODISSECTION; CARCINOMA-IN-SITU; FOLLICLE DEVELOPMENT; W-LOCUS; MOUSE; LIGAND; PROTEIN; GENE; SPERMATOGENESIS; SPERMATOGONIA;
Keywords:
c-kit; gonocyte; human; oocyte; laser capture microscopy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Anderson, RA MRC, Human Reprod Sci Unit, 37 Chalmers St, Edinburgh EH3 9ET, Midlothian,Scotland MRC 37 Chalmers St Edinburgh Midlothian Scotland EH3 9ET land
Citazione:
L.L.L. Robinson et al., "Germ cell specific expression of c-kit in the human fetal gonad", MOL HUM REP, 7(9), 2001, pp. 845-852

Abstract

The proto-oncogene receptor, e-kit, and its ligand have been demonstrated to be essential to the processes of germ cell migration, proliferation and survival in the rodent. The aim of the present study was to investigate theexpression of c-kit mRNA and protein in human fetal ovary and testis across the gestational period 13-21 weeks. In the ovary, this crucial period of development spans the transition from oogonial replication by mitosis to primordial follicle formation. In the testis, germ cells (gonocytes) are mitotically active. Expression of c-kit mRNA was demonstrated by reverse transcription-polymerase chain reaction (RT-PCR) in both ovary and testis at all gestational ages examined. Testicular germ cell specific expression of c-kit mRNA was confirmed by RT-PCR using specific cell types recovered by lasercapture microscopy. The expression of c-kit protein by both male and female germ cells was demonstrated by immunohistochemistry at all gestational ages examined, and was confirmed by immunoblotting. In both, c-kit was localized to the cell membrane except in oocytes within primordial follicles where it was localized to the cytoplasm. These data demonstrate that the expression of c-kit mRNA and protein is germ cell specific in human fetal gonads and are consistent with an important role for the c-kit/kit ligand signalling system in germ cell proliferation and survival in the developing human gonad.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 09:14:04