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Titolo:
Large-scale production of a therapeutic protein in transgenic tobacco plants: effect of subcellular targeting on quality of a recombinant dog gastriclipase
Autore:
Gruber, V; Berna, PP; Arnaud, T; Bournat, P; Clement, C; Mison, D; Olagnier, B; Philippe, L; Theisen, M; Baudino, S; Benicourt, C; Cudrey, C; Bloes, C; Duchateau, N; Dufour, S; Gueguen, C; Jacquet, S; Ollivo, C; Poncetta, C; Zorn, N; Ludevid, D; Van Dorsselaer, A; Verger, R; Doherty, A; Merot, B; Danzin, C;
Indirizzi:
Meristem Therapeut, F-63100 Clermont Ferrand, France Meristem Therapeut Clermont Ferrand France F-63100 rmont Ferrand, France Inst Rech Jouveinal Parke Davis, F-94265 Fresnes, France Inst Rech Jouveinal Parke Davis Fresnes France F-94265 5 Fresnes, France Univ Strasbourg 1, CNRS, UMR 7509, F-67008 Strasbourg, France Univ Strasbourg 1 Strasbourg France F-67008 , F-67008 Strasbourg, France CSIC, ES-08034 Barcelona, Spain CSIC Barcelona Spain ES-08034CSIC, ES-08034 Barcelona, Spain Lab Lipolyse Enzymat, CNRS, UPR 9025, F-13402 Marseille, France Lab Lipolyse Enzymat Marseille France F-13402 F-13402 Marseille, France
Titolo Testata:
MOLECULAR BREEDING
fascicolo: 4, volume: 7, anno: 2001,
pagine: 329 - 340
SICI:
1380-3743(2001)7:4<329:LPOATP>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROMOTER SEQUENCES; PURIFICATION; EXPRESSION; ANTIGEN; CELLS; ANTIBODIES; VIRUS; GENES;
Keywords:
dog gastric lipase; open-field production; signal sequence; subcellular localization; therapeutic protein; transgenic tobacco;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Gruber, V Meristem Therapeut, 8 Rue Freres Lumiere,ZI du Brezet, F-63100 Clermont Ferrand, France Meristem Therapeut 8 Rue Freres Lumiere,ZI du Brezet Clermont Ferrand France F-63100
Citazione:
V. Gruber et al., "Large-scale production of a therapeutic protein in transgenic tobacco plants: effect of subcellular targeting on quality of a recombinant dog gastriclipase", MOL BREED, 7(4), 2001, pp. 329-340

Abstract

A recombinant dog gastric lipase with therapeutic potential for the treatment of exocrine pancreatic insufficiency was expressed in transgenic tobacco plants. We targeted the protein using two different signal sequences for either vacuolar retention or secretion. In both cases, an active glycosylated recombinant protein was obtained. The recombinant enzymes and the nativeenzyme displayed similar properties including acid resistance and acidic optimum pH. The proteolytic maturation and the specific activity of the recombinant proteins, however, were found to be dependent on subcellular compartmentalization. Expression levels of recombinant dog gastric lipase were about 5% and 7% of acid extractable plant proteins for vacuolar retention andsecretion respectively. This expression system already has allowed the production of tens of grams of purified lipase through open-field culture of transgenic tobacco plants.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 01:29:52