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Titolo:
The candidate gene approach in plant genetics: a review
Autore:
Pflieger, S; Lefebvre, V; Causse, M;
Indirizzi:
INRA, Unite Genet & Ameliorat Fruits & Legumes, F-84143 Montfavet, France INRA Montfavet France F-84143 ruits & Legumes, F-84143 Montfavet, France
Titolo Testata:
MOLECULAR BREEDING
fascicolo: 4, volume: 7, anno: 2001,
pagine: 275 - 291
SICI:
1380-3743(2001)7:4<275:TCGAIP>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
QUANTITATIVE TRAIT LOCI; DISEASE-RESISTANCE GENES; CYSTIC-FIBROSIS GENE; EXPRESSED SEQUENCE TAGS; NUCLEOTIDE-BINDING SITE; ARABIDOPSIS-THALIANA; MILDEW RESISTANCE; BRASSICA-NAPUS; TOMATO FRUIT; DNA CHIPS;
Keywords:
candidate gene; gene cloning; gene mapping; Mendelian trait locus; quantitative trait locus;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
139
Recensione:
Indirizzi per estratti:
Indirizzo: Lefebvre, V INRA, Unite Genet & Ameliorat Fruits & Legumes, Domaine St Maurice BP 94, F-84143 Montfavet, France INRA Domaine St Maurice BP 94 Montfavet France F-84143 France
Citazione:
S. Pflieger et al., "The candidate gene approach in plant genetics: a review", MOL BREED, 7(4), 2001, pp. 275-291

Abstract

The candidate gene (CG) approach has been applied in plant genetics in thepast decade for the characterisation and cloning of Mendelian and quantitative trait loci (QTLs). It constitutes a complementary strategy to map-based cloning and insertional mutagenesis. The goal of this paper is to presentan overview of CG analyses in plant genetics. CG analysis is based on the hypothesis that known-function genes (the candidate genes) could correspondto loci controlling traits of interest. CGs refer either to cloned genes presumed to affect a given trait (`functional CGs') or to genes suggested bytheir close proximity on linkage maps to loci controlling the trait (`positional CGs'). In plant genetics, the most common way to identify a CG is tolook for map co-segregation between CGs and loci affecting the trait. Statistical association analyses between molecular polymorphisms of the CG and variation in the trait of interest have also been carried out in a few studies. The final validation of a CG will be provided through physiological analyses, genetic transformation and/or sexual complementation. Theoretical and practical applications of validated CGs in plant genetics and breeding are discussed.

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Documento generato il 13/07/20 alle ore 10:13:33