Catalogo Articoli (Spogli Riviste)

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Titolo:
Caspase activity is involved in, but is dispensable for, early motoneuron death in the chick embryo cervical spinal cord
Autore:
Yaginuma, H; Shiraiwa, N; Shimada, T; Nishiyama, K; Hong, J; Wang, SW; Momoi, T; Uchiyama, Y; Oppenheim, RW;
Indirizzi:
Fukushima Med Univ, Sch Med, Dept Anat, Fukushima 9601295, Japan FukushimaMed Univ Fukushima Japan 9601295 nat, Fukushima 9601295, Japan Wake Forest Univ, Bowman Gray Sch Med, Dept Neurobiol & Anat, Winston Salem, NC 27157 USA Wake Forest Univ Winston Salem NC USA 27157 , Winston Salem, NC 27157 USA Wake Forest Univ, Bowman Gray Sch Med, Program Neurosci, Winston Salem, NC27157 USA Wake Forest Univ Winston Salem NC USA 27157 i, Winston Salem, NC27157 USA NCNP, Natl Inst Neurosci, Div Dev & Differentiat, Tokyo 1878502, Japan NCNP Tokyo Japan 1878502 i, Div Dev & Differentiat, Tokyo 1878502, Japan Osaka Univ, Sch Med, Dept Cell Biol & Anat 1, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 Anat 1, Suita, Osaka 5650871, Japan Univ Tsukuba, Inst Clin Med, Dept Neurol, Tsukuba, Ibaraki 3058575, Japan Univ Tsukuba Tsukuba Ibaraki Japan 3058575 sukuba, Ibaraki 3058575, Japan
Titolo Testata:
MOLECULAR AND CELLULAR NEUROSCIENCE
fascicolo: 2, volume: 18, anno: 2001,
pagine: 168 - 182
SICI:
1044-7431(200108)18:2<168:CAIIIB>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRAMMED CELL-DEATH; SITE-DIRECTED ANTISERUM; DNA FRAGMENTATION; GRANZYME-B; IN-VIVO; INDUCED APOPTOSIS; ACTIVATED CASPASE-3; PROTEASE CASCADE; NEURONAL DEATH; NERVOUS-SYSTEM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
65
Recensione:
Indirizzi per estratti:
Indirizzo: Yaginuma, H Fukushima Med Univ, Sch Med, Dept Anat, Fukushima 9601295, Japan Fukushima Med Univ Fukushima Japan 9601295 ma 9601295, Japan
Citazione:
H. Yaginuma et al., "Caspase activity is involved in, but is dispensable for, early motoneuron death in the chick embryo cervical spinal cord", MOL CELL NE, 18(2), 2001, pp. 168-182

Abstract

We examined the role of caspases in the early programmed cell death (PCD) of motoneurons (MNs) in the chick embryo cervical cord between embryonic day (E) 4 and E5. An increase in caspase-3-like activity in MNs was observed at E4.5. Treatment with an inhibitor of caspase-3-like activity, Ac-DEVD-CHO, for 12 h blocked this increase and revealed that caspase-3-like activityis mainly responsible for DNA fragmentation and the nuclear changes duringPCD but not for degenerative changes in the cytoplasm. When a more broad-spectrum caspase inhibitor was used (bocaspartyl (OMe)-fluoromethyl ketone, BAF), the appearance of degenerative changes in the cytoplasm was delayed by at least 12 h. However, following treatment with either Ac-DEVD-CHO or BAF for 24 h, the number of surviving healthy MNs did not differ from controls, indicating a normal occurrence of PCD despite the inhibition of caspases. These results suggest that caspase cascades that occur upstream of and are independent of the activation of caspase-3-like activity are responsible for the degenerative changes in the cytoplasm of dying cervical MNs. These data also suggest that, although one function of caspases may be to facilitate the kinetics of PCD, caspases are nonetheless dispensable for at least some forms of normal neuronal PCD in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 08:42:22