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Titolo:
The dynamics of SAP90/PSD-95 recruitment to new synaptic junctions
Autore:
Bresler, T; Ramati, Y; Zamorano, PL; Zhai, R; Garner, CC; Ziv, NE;
Indirizzi:
Technion Israel Inst Technol, Rappaport Family Inst Res Med Sci, IL-31096 Haifa, Israel Technion Israel Inst Technol Haifa Israel IL-31096 L-31096 Haifa, Israel Technion Israel Inst Technol, Bruce Rappaport Fac Med, Dept Anat & Cell Biol, IL-31096 Haifa, Israel Technion Israel Inst Technol Haifa Israel IL-31096 L-31096 Haifa, Israel Univ Alabama, Dept Neurobiol, Birmingham, AL USA Univ Alabama Birmingham AL USA abama, Dept Neurobiol, Birmingham, AL USA
Titolo Testata:
MOLECULAR AND CELLULAR NEUROSCIENCE
fascicolo: 2, volume: 18, anno: 2001,
pagine: 149 - 167
SICI:
1044-7431(200108)18:2<149:TDOSRT>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
POSTSYNAPTIC DENSITY FRACTION; PRESYNAPTIC ACTIVE ZONES; SUPPRESSOR GENE DLG; HIPPOCAMPAL-NEURONS; EXCITATORY SYNAPSES; NMDA RECEPTOR; MOLECULAR DETERMINANTS; GLUTAMATE RECEPTORS; AMPA RECEPTORS; PSD-95;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Bresler, T Technion Israel Inst Technol, Rappaport Family Inst Res Med Sci, IL-31096 Haifa, Israel Technion Israel Inst Technol Haifa Israel IL-31096 fa, Israel
Citazione:
T. Bresler et al., "The dynamics of SAP90/PSD-95 recruitment to new synaptic junctions", MOL CELL NE, 18(2), 2001, pp. 149-167

Abstract

SAP90/PSD-95 is thought to be a central organizer of the glutamatergic synapse postsynaptic reception apparatus. To assess its potential role during glutamatergic synapse formation, we used GFP-tagged SAP90/PSD-95, time lapse confocal microscopy, and cultured hippocampal neurons to determine its dynamic recruitment into new synaptic junctions. We report that new SAP90/PSD-95 clusters first appeared at new axodendritic contact sites within 20-60 min of contact establishment. SAP90/PSD-95 clustering was rapid, with kinetics that fit a single exponential with a mean time constant of similar to 23 min. Most new SAP90/PSD-95 clusters were found juxtaposed to functional presynaptic boutons as determined by labeling with FM 4-64. No evidence was found for the existence of discrete transport particles similar to those previously reported to mediate presynaptic active zone cytoskeleton assembly. Instead, we found that SAP90/PSD-95 is recruited to nascent synapses from a diffuse dendritic cytoplasmic pool. Our findings show that SAP90/PSD-95 is recruited to nascent synaptic junctions early during the assembly processand indicate that its assimilation is fundamentally different from that ofpresynaptic active zone components.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 21:38:39