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Titolo:
Evaluation of inner ear gene transfer following middle ear application of an adenoviral vector.
Autore:
Stover, T; Kawamoto, K; Kanzaki, S; Raphael, Y;
Indirizzi:
Hannover Med Sch, Hals Nasen Ohrenklin, D-30625 Hannover, Germany HannoverMed Sch Hannover Germany D-30625 lin, D-30625 Hannover, Germany Univ Michigan, Sch Med, Kresge Hearing Res Inst, Otophathol Lab, Ann Arbor, MI USA Univ Michigan Ann Arbor MI USA s Inst, Otophathol Lab, Ann Arbor, MI USA
Titolo Testata:
LARYNGO-RHINO-OTOLOGIE
fascicolo: 8, volume: 80, anno: 2001,
pagine: 431 - 435
SICI:
1615-0007(200108)80:8<431:EOIEGT>2.0.ZU;2-W
Fonte:
ISI
Lingua:
GER
Soggetto:
GUINEA-PIG; IN-VIVO; ROUND-WINDOW; NEUROTROPHIC FACTOR; COCHLEAR; OTOSCLEROSIS; COCHLEOSTOMY; DEGENERATION; OTOTOXICITY; GENTAMICIN;
Keywords:
cochlea; adenovirus; gene transfer; middle ear; round window membrane; stapes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Stover, T Hannover Med Sch, Hals Nasen Ohrenklin, Carl Neuberg Str 1, D-30625 Hannover, Germany Hannover Med Sch Carl Neuberg Str 1 Hannover GermanyD-30625 ny
Citazione:
T. Stover et al., "Evaluation of inner ear gene transfer following middle ear application of an adenoviral vector.", LARY RH OTO, 80(8), 2001, pp. 431-435

Abstract

Background: Several groups demonstrated in animal experiments that gene transfer is a feasible tool for inner ear intervention. Various approaches for inoculation of vectors have been successfully used for inner ear gene therapy. One possible way to reduce the risk of hearing loss following the opening of the cochlea for application of the vector into the perilymphatic space is to deliver vectors through the round window. This study was designedto determine whether middle ear application of an adenoviral vector is a feasible approach to inoculate vectors and lead to transduction of cells in the inner ear. Methods: A unilateral middle ear application of an adenoviral vector was performed in 4 guinea pigs directly on the round window membrane (RWM) and in 4 additional animals by placing a cotton patch soaked with the vector solution on the RWM. The expression of a reporter gene (lacZ) was used to localize vector-transcluced cells. Results: Only one out of 8 animals showed cochlear expression of the reporter gene, whereas all 8 animalsshowed strong lacZ expression in the middle ear mucosa, in the RWM and in the mucosa surrounding the stapes. Conclusion: Our results indicate that the RWM presents a close barrier, almost completely preventing the adenovirusto diffuse into the perilymphatic space. Therefore middle ear application of an adenoviral vector cannot be used to induce inner ear gene transfer. However, middle ear application of a viral vector may be useful for developing treatment for diseases of the middle ear mucosa.

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Documento generato il 22/01/20 alle ore 12:46:34