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Titolo:
Identification and characterization by high-performance liquid chromatography/electrospray ionization mass spectrometry of a new variant hemoglobin, Mataro [beta 134(H12) Val > Ala]
Autore:
Gallardo, AM; Hernandez, JA; Hernandez, JM; Rovira, JM; Fluvia, L; Bosch, A; Pastor, MC;
Indirizzi:
Fundacio Recerca Biomed Germans Trias & Pujol, Barcelona 08916, Spain Fundacio Recerca Biomed Germans Trias & Pujol Barcelona Spain 08916 pain Hosp Mataro, Serv Hematol, Mataro, Spain Hosp Mataro Mataro SpainHosp Mataro, Serv Hematol, Mataro, Spain Hosp Univ HUGTIP, Barcelona, Spain Hosp Univ HUGTIP Barcelona SpainHosp Univ HUGTIP, Barcelona, Spain
Titolo Testata:
JOURNAL OF MASS SPECTROMETRY
fascicolo: 8, volume: 36, anno: 2001,
pagine: 943 - 949
SICI:
1076-5174(200108)36:8<943:IACBHL>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLLISION-INDUCED DISSOCIATION; SICKLE-CELL DISEASE; ELECTROSPRAY-IONIZATION; TRYPTIC PEPTIDES; HIS->TYR; IONS;
Keywords:
liquid chromatography/electrospray ionization mass spectrometry; human hemoglobin variant; collision-induced dissociation; peptide sequencing;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Gallardo, AM Fundacio Recerca Biomed Germans Trias & Pujol, Crta Canyet S-N, Barcelona 08916, Spain Fundacio Recerca Biomed Germans Trias & Pujol Crta Canyet S-N Barcelona Spain 08916
Citazione:
A.M. Gallardo et al., "Identification and characterization by high-performance liquid chromatography/electrospray ionization mass spectrometry of a new variant hemoglobin, Mataro [beta 134(H12) Val > Ala]", J MASS SPEC, 36(8), 2001, pp. 943-949

Abstract

This work illustrates the practical use of combined microbore reversed-phase high-performance liquid chromatography (RP-HPLC) with electrospray ionization mass spectrometry (ESI-MS) in protein identification. The approach consisted of the detection of the abnormal beta -globin chain by ESI-MS analysis of mixtures of intact globins, which simultaneously provided their molecular masses. Separation of the polypeptide globin chains was carried out using microbore C-4 RP-HPLC on-line with ESI-MS. Direct peptide-mapping ESI-MS without previous chromatographic separation was performed in order to identify tryptic peptides from whole blood. For the sequence confirmation of the abnormal peptide containing the mutation point, C-18 RP-HPLC tryptic separation of the globin mixture on-line with collision-induced dissociation (CID) fragmentation was done. The y series ions allowed the identification of the hemoglobin (Hb) variant as [beta 134(H12) Val > Ala]. This new Hb was named Rb Matar6, after the city where it was detected. Copyright (C) 2001John Wiley & Sons, Ltd.

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Documento generato il 02/04/20 alle ore 19:10:16