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Titolo:
Stable isotope dilution negative ion chemical ionization gas chromatography-mass spectrometry for the quantitative analysis of paroxetine in human plasma
Autore:
Leis, HJ; Windischhofer, W; Raspotnig, G; Fauler, G;
Indirizzi:
Graz Univ, Childrens Hosp, Div Analyt Biochem & Mass Spectrometry, A-8036 Graz, Austria Graz Univ Graz Austria A-8036 & Mass Spectrometry, A-8036 Graz, Austria
Titolo Testata:
JOURNAL OF MASS SPECTROMETRY
fascicolo: 8, volume: 36, anno: 2001,
pagine: 923 - 928
SICI:
1076-5174(200108)36:8<923:SIDNIC>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; SPARTEINE OXIDATION POLYMORPHISM; INTERNAL STANDARD; METABOLITES; BLOOD;
Keywords:
paroxetine; NICI; GC-MS; stable isotope dilution;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Leis, HJ Graz Univ, Childrens Hosp, Div Analyt Biochem & Mass Spectrometry, Auenbruggerpl 30, A-8036 Graz, Austria Graz Univ Auenbruggerpl 30 Graz Austria A-8036 036 Graz, Austria
Citazione:
H.J. Leis et al., "Stable isotope dilution negative ion chemical ionization gas chromatography-mass spectrometry for the quantitative analysis of paroxetine in human plasma", J MASS SPEC, 36(8), 2001, pp. 923-928

Abstract

A sensitive and specific method for the quantitative determination of paroxetine in human plasma is presented. After solvent extraction from plasma with hexane/ethyl acetate (1:1) at alkaline pH and derivatization to the pentafluorobenzyl carbamate derivative, paroxetine was measured by gas chromatography-negative ion chemical ionization mass spectrometry. The carboxylateanion at m/z 372 was obtained at high relative abundance. [H-2(6)]-labeledparoxetine was used as an internal standard and its rapid and facile preparation from the unlabeled compound is described. Calibration graphs were linear within a range of 0.094-12.000 ng ml(-1) using 1 ml of plasma and 0.469-60 ng ml-1 using 200 mul of plasma. Intra-day precision was 1.47% (0.375 ng ml(-1)), 3.16% (3 ng ml(-1)) and 1.37% (9 ng ml(-1)) for the low-level method, and 3.37% (1.875 ng ml(-1)), 2.72% (15 ng ml(-1)) and 2.22% (45 ng ml(-1)) for the high-level method. Inter-day precision was 1.65% (0.375 ng ml(-1)), 2.13% (3 ng ml(-1)) and 1.66% (9 ng ml(-1)) for the low-level method, and 1.10% (1.875 ng ml(-1)), 1.56% (15 ng ml(-1)) and 1.90% (45 ng ml(-1)) for the high-level method. At the limit of quantification (0.094 ng ml(-1)), intra-day precision was 4.30% (low-level method) and 2.56% (high-levelmethod), and inter-day precision was 3.23% (low-level method) and 3.00% (high-level method). The method is rugged, rapid and robust and has been applied to the batch analysis of paroxetine during pharmacokinetic profiling ofthe drug. Copyright (C) 2001 John Wiley & Sons, Ltd.

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Documento generato il 18/01/20 alle ore 21:30:22