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Titolo:
Simultaneous determination of all-trans, 9-cis, 13-cis retinoic acid and retinol in rat prostate using liquid chromatography-mass spectrometry
Autore:
Wang, Y; Chang, WL; Prins, GS; van Breemen, RB;
Indirizzi:
Univ Illinois, Coll Pharm, Dept Med Chem & Pharmacognosy, Chicago, IL 60612 USA Univ Illinois Chicago IL USA 60612 & Pharmacognosy, Chicago, IL 60612 USA Univ Illinois, Dept Urol, Chicago, IL USA Univ Illinois Chicago IL USAUniv Illinois, Dept Urol, Chicago, IL USA
Titolo Testata:
JOURNAL OF MASS SPECTROMETRY
fascicolo: 8, volume: 36, anno: 2001,
pagine: 882 - 888
SICI:
1076-5174(200108)36:8<882:SDOA91>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
QUANTIFICATION;
Keywords:
retinoic acid; retinol; prostate; LC-MS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: van Breemen, RB Univ Illinois, Coll Pharm, Dept Med Chem & Pharmacognosy, 833 S Wood St, Chicago, IL 60612 USA Univ Illinois 833 S Wood St Chicago ILUSA 60612 60612 USA
Citazione:
Y. Wang et al., "Simultaneous determination of all-trans, 9-cis, 13-cis retinoic acid and retinol in rat prostate using liquid chromatography-mass spectrometry", J MASS SPEC, 36(8), 2001, pp. 882-888

Abstract

Since retinoic acid (RA) and RA receptors are key developmental regulatorsduring organogenesis, they might participate in the abnormal development of the prostate caused by early estrogen exposure. In order to test this assumption, a sensitive analytical method that can differentiate 9-cis, 13-cis, and all-trans RA in small tissue samples (similar to8 mg) is required. Since retinol is the metabolic precursor to RA, simultaneous quantification of retinol would also provide valuable information. Here, we report a liquidchromatography-mass spectrometry method for simultaneous determination of retinol and 9-cis, 13-cis, and all-trans RA in rat prostate. Mass spectrometric signal responses for RA were compared using positive ion atmospheric-pressure chemical ionization (APCI) and electrospray, as well as positive ion and negative ion APCI. Positive ion APCI was selected for all subsequent analysis for its better sensitivity, and to provide simultaneous determination of retinol and RA. Ventral prostate tissue samples were homogenized andextracted following simple protein precipitation without derivatization. Baseline separation of 9-cis, 13-cis, and all-trans RA standards was obtained by using a non-porous silica C-18 column. Selected ion monitoring of the ions m/z 301 and m/z 269 was carried out for mass spectrometric quantitative analysis. The ion of m/z 301 corresponded to the protonated molecule of RA, whereas the ion of m/z 269 corresponded to loss of water or acetic acid from the protonated molecule of retinol or the internal standard retinyl acetate respectively. The method has a linear response over a concentration range of at least three orders of magnitude. The limit of quantitation was determined to be 702 fmol all-trans RA injected on-column. The method showedexcellent intra- and inter-assay reproducibility and good recovery, and issuitable for analyzing RA and retinol in small tissue samples (similar to8mg). Copyright (C) 2001 John Wiley & Sons, Ltd.

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Documento generato il 22/01/20 alle ore 07:22:52