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Titolo:
Subcutaneous administration of interleukin-2 triggers Fc gamma receptor I expression on human peripheral blood neutrophils in solid and hematologic malignancies
Autore:
Sconocchia, G; Cococcetta, NY; Campagnano, L; Amadori, S; Iorio, B; Boffo, V; Ferdinandi, V; Del Principe, I; Adorno, D; Casciani, CU;
Indirizzi:
CNR, Inst Tissue Typing & Dialysis, Rome, Italy CNR Rome ItalyCNR, Inst Tissue Typing & Dialysis, Rome, Italy Univ Roma Tor Vergata, Dept Surg, Rome, Italy Univ Roma Tor Vergata RomeItaly ma Tor Vergata, Dept Surg, Rome, Italy Univ Roma Tor Vergata, Dept Biopathol, Rome, Italy Univ Roma Tor Vergata Rome Italy r Vergata, Dept Biopathol, Rome, Italy Ctr Traumatol Ortoped, Rome, Italy Ctr Traumatol Ortoped Rome ItalyCtr Traumatol Ortoped, Rome, Italy
Titolo Testata:
JOURNAL OF IMMUNOTHERAPY
fascicolo: 4, volume: 24, anno: 2001,
pagine: 374 - 383
SICI:
1524-9557(200107/08)24:4<374:SAOITF>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN POLYMORPHONUCLEAR LEUKOCYTES; COLONY-STIMULATING FACTOR; NECROSIS-FACTOR-ALPHA; RENAL-CELL CARCINOMA; INTERFERON-ALPHA; MYELOID CELLS; SERUM LEVELS; IGG; IL-2; GRANULOCYTES;
Keywords:
IL-2; immunotherapy; Fc gamma RI; MNs; malignancies;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Sconocchia, G NHLBI, Allogene Stem Cell Transplantat Sect, Hematol Branch,NIH, Bldg 10,Room 7C103,9000 Rockville Pike, Bethesda, MD 20892 USA NHLBI Bldg 10,Room 7C103,9000 Rockville Pike Bethesda MD USA 20892
Citazione:
G. Sconocchia et al., "Subcutaneous administration of interleukin-2 triggers Fc gamma receptor I expression on human peripheral blood neutrophils in solid and hematologic malignancies", J IMMUNOTH, 24(4), 2001, pp. 374-383

Abstract

Freshly isolated human polymorphonuclear cells (PMNCs) constitutively express Fc gamma receptor (Fc gammaR) II and Fc gamma RIII on the cell surface but not Fc gamma RI. Cytokines such as interferon-gamma (IFN-gamma), granulocyte-macrophage colony-stimulating factor (CSF), and granulocyte-CSF trigger Fc gamma RI expression on (PMNCs). Because PMNCs express interleukin (IL)-2 receptor, we investigated whether IL-2 can induce Fc gamma RI expression on PMNCs isolated from IL-2-treated metastatic renal cell carcinoma (MRCC) and low-grade non-Hodgkin lymphoma (LGNHL) patients. Pretherapy flow cytometry analysis of Fc receptors on PMNCs did not show Fc gamma RI expression. Interestingly, 3 days after therapy, PMNCs displayed a detectable amount of Fc gamma RI on the cell surface. Kinetic studies on the in vivo effects of IL-2 on MRCC patients showed that Fc gamma RI was transiently expressed,starting within 3-6 days of therapy, remaining expressed for 10-15 days, and rapidly declining, whereas such expression remained stable for months inLGNHL patients. In contrast, Fc gamma RII was not affected. In addition, Fc gamma RI+ PMNCs coated in vitro with a bispecific antibody Fab anti-Fc gamma RI x anti-HER-2/neu formed intercellular conjugates with a human HER-2/neu-transrected 3T3 cell line (HER-2/neu-3T3). Interleukin-2 treatment increased the number of Fc gamma RIII low eosinophils, leading to a change in Fc-yRIII distribution among granulocyte cell subsets. In vitro IL-2 treatment of purified PMNCs failed to generate Fc gamma RI expression, suggesting that IL-2 indirectly causes Fc gamma RI expression. During the IL-2 administration, we did not observe significant changes in IFN gamma serum level. Inconclusion, our observation may be used to potentiate the antitumor effects of IL-2 in novel immunotherapy regimens, perhaps by redirecting Fc gamma RI+ PMNCs against cancer cells by heteroconjugate antibodies and monitoringthe biologic activity of subcutaneous IL-2 in cancer patients.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 00:23:13