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Titolo:
Adenovirus-mediated MUC1 gene transduction into human blood-derived dendritic cells
Autore:
Maruyama, K; Akiyama, Y; Nara-Ashizawa, N; Hojo, T; Cheng, JY; Mizuguchi, H; Hayakawa, T; Yamaguchi, K;
Indirizzi:
Natl Inst Hlth Sci, Div Biol Chem & Biol, Tokyo 158, Japan Natl Inst Hlth Sci Tokyo Japan 158 iv Biol Chem & Biol, Tokyo 158, Japan Natl Canc Ctr, Res Inst, Div Growth Factor, Chuo Ku, Tokyo 1040045, Japan Natl Canc Ctr Tokyo Japan 1040045 Factor, Chuo Ku, Tokyo 1040045, Japan
Titolo Testata:
JOURNAL OF IMMUNOTHERAPY
fascicolo: 4, volume: 24, anno: 2001,
pagine: 345 - 353
SICI:
1524-9557(200107/08)24:4<345:AMGTIH>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-ANTIGEN MUC1; MAJOR HISTOCOMPATIBILITY COMPLEX; CYTOTOXIC T-LYMPHOCYTES; TANDEM REPEAT PEPTIDE; IN-VITRO; ANTITUMOR IMMUNITY; RECOMBINANT ADENOVIRUS; PROTEIN; GLYCOSYLATION; RESPONSES;
Keywords:
human blood DC; adenovirus vector; MUC1; mixed leukocyte reaction; cytotoxic T-lymphocyte;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Akiyama, Y Natl Canc Ctr, Res Inst, Div Growth Factor, Chuo Ku, 5-1-1 Tsukiji, Tokyo 1040045, Japan Natl Canc Ctr 5-1-1 Tsukiji Tokyo Japan 1040045 1040045, Japan
Citazione:
K. Maruyama et al., "Adenovirus-mediated MUC1 gene transduction into human blood-derived dendritic cells", J IMMUNOTH, 24(4), 2001, pp. 345-353

Abstract

MUC1 protein is widely expressed on various human cancer cells and has a specific highly glycosylated core structure with multiple tandem repeats, which may include an immunogenic peptide sequence. The potency of MUC1 protein to induce human histocompatibility leukocyte antigen-class I-restricted cytotoxic T-lymphocyte (CTL) induction remains to be fully clarified in human beings. In the current study, we made MUC1-expressing human dendritic cells (DCs) using recombinant adenovirus vector. Adenovirus vector plasmid containing human MUC1 cDNA, pAdHM4-MUC1 was constructed using in vitro ligation with a shuttle vector, pHMCMV5. Adenovirus vector expressing MUC1 was generated by the transfection of PacI-digested recombinant vector plasmid into293 cells. Human blood DCs were obtained front 7-day culture of monocytes with recombinant human (rh) granulocyte-macrophage (GM) colony-stimulating factor (CSF) and (rh)interleukin (IL)-4. Then, 1 x 10(6) DCs were incubatedwith viral supernatant at a multiplicity of infection of 200 for 24 It in the presence of rhGM-CSF and rhIL-4. Flow cytometric analysis showed that 30% to 40% of the transduced DCs expressed MUC1 proteins by contrast, nontransduced or transduced DCs with mock virus expressed only small amounts of MUC1 protein. Adenovirus-mediated MUC1 gene transduction into DCs had no significant effect on DC surface marker expressions or functions such as mixedleukocyte reaction. Furthermore, MUC1-specific CD8(+) CTLs could be induced from healthy donor blood lymphocytes using MUC1-expressing DCs as stimulators. These results suggested that MUC1 gene-transduced DCs are a functional and potent tool for triggering a CTL response against MUC1(+) cancer cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 20:43:00