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Titolo:
Major histocompatibility complex-restricted lysis of neuroblastoma cells by autologous cytotoxic T lymphocytes
Autore:
Sarkar, AK; Burlingame, SM; Zang, YQ; Dulai, V; Hicks, MJ; Strother, DR; Nuchtern, JG;
Indirizzi:
Texas Childrens Hosp, DeBakey Dept Surg, Houston, TX 77030 USA Texas Childrens Hosp Houston TX USA 77030 ept Surg, Houston, TX 77030 USA Texas Childrens Hosp, Baylor Coll Med, Dept Pathol, Houston, TX 77030 USA Texas Childrens Hosp Houston TX USA 77030 t Pathol, Houston, TX 77030 USA Texas Childrens Hosp, Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA Texas Childrens Hosp Houston TX USA 77030 t Pediat, Houston, TX 77030 USA Texas Childrens Hosp, Texas Childrens Canc Ctr, Houston, TX 77030 USA Texas Childrens Hosp Houston TX USA 77030 Canc Ctr, Houston, TX 77030 USA
Titolo Testata:
JOURNAL OF IMMUNOTHERAPY
fascicolo: 4, volume: 24, anno: 2001,
pagine: 305 - 311
SICI:
1524-9557(200107/08)24:4<305:MHCLON>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
NATURAL-KILLER-CELLS; NEURO-BLASTOMA; TUMOR-CELLS; LINES; CHILDREN; INTERLEUKIN-2;
Keywords:
children; neuroblastoma; cancer; immunotherapy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Nuchtern, JG Div Pediat Surg, MC3-2325,6621 Fannin, Houston, TX 77030 USA Div Pediat Surg MC3-2325,6621 Fannin Houston TX USA 77030 USA
Citazione:
A.K. Sarkar et al., "Major histocompatibility complex-restricted lysis of neuroblastoma cells by autologous cytotoxic T lymphocytes", J IMMUNOTH, 24(4), 2001, pp. 305-311

Abstract

Vigorous host immune reactivity to neuroblastoma may correlate with betterprognosis, but identification of human cytotoxic T-lymphocyte (CTL) responses has been relatively unsuccessful. We generated neuroblastoma-reactive CTL lines from two human leukocyte antigen (HLA) A2(+) neuroblastoma patients by stimulation of peripheral blood lymphocytes (PBLs) with irradiated autologous tumor cells pretreated with interferon-gamma in the presence of lowconcentrations of interleukin-2 (5 U/mL). These lines lyse autologous tumor cells but do not kill HLA mismatched allogeneic tumor cells, Epstein-Barrvirus-transformed autologous B cells, or standard natural killer cell targets. Cytotoxic T lymphocytes generated from one patient recognize tumor cells from several HLA-A2 matched children, although the other patient's CTLs do not kill tumor cells from other HLA-A2(+) individuals. Pretreatment of CTLs or target cells with appropriate standard monoclonal antibodies demonstrates that these CTLs are major histocompatibility complex class I (HLA-A2)restricted and that the effector cell population is CD8(+). Our findings suggest that these tumor cells express at least one common HLA-A2 restrictedantigen and at least one unique private epitope. Autologous tumor-specificCTLs can be readily generated front patients' PBLs and maintained in long-term culture using standard techniques.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 00:10:14