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Titolo:
Normal T-cell response and in vivo magnetic resonance imaging of T cells loaded with HIV transactivator-peptide-derived superparamagnetic nanoparticles
Autore:
Dodd, CH; Hsu, HC; Chu, WJ; Yang, PG; Zhang, HG; Mountz, JD; Zinn, K; Forder, J; Josephson, L; Weissleder, R; Mountz, JM; Mountz, JD;
Indirizzi:
Univ Alabama, Dept Med, Div Clin Immunol & Rheumatol, Birmingham, AL 35294USA Univ Alabama Birmingham AL USA 35294 & Rheumatol, Birmingham, AL 35294USA Univ Alabama, Dept Radiol, Ctr Nucl Imaging Res, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 maging Res, Birmingham, AL 35294 USA Univ Alabama, Dept Radiol, Div Nucl Med, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 v Nucl Med, Birmingham, AL 35294 USA Birmingham Vet Adm Med Ctr, Birmingham, AL 35294 USA Birmingham Vet Adm Med Ctr Birmingham AL USA 35294 rmingham, AL 35294 USA Massachusetts Gen Hosp, Ctr Mol Imaging Res, Boston, MA 02129 USA Massachusetts Gen Hosp Boston MA USA 02129 ging Res, Boston, MA 02129 USA
Titolo Testata:
JOURNAL OF IMMUNOLOGICAL METHODS
fascicolo: 1-2, volume: 256, anno: 2001,
pagine: 89 - 105
SICI:
0022-1759(20011001)256:1-2<89:NTRAIV>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
TAT PROTEIN; IN-VIVO; ADHESION MOLECULES; HIGH-RESOLUTION; APOPTOSIS; ACTIVATION; EXPRESSION; MICE; LYMPHOCYTES; ANTIGEN;
Keywords:
rodent; T lymphocytes; cell trafficking; MRI, superparamagnetic iron oxide; homing; in vivo animal models;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Mountz, JD Univ Alabama, Dept Med, Div Rheumatol, 701 S 19th St,473 Lyons Harrison Res Bldg, Birmingham, AL 35294 USA Univ Alabama 701 S 19th St,473 Lyons Harrison Res Bldg Birmingham AL USA 35294
Citazione:
C.H. Dodd et al., "Normal T-cell response and in vivo magnetic resonance imaging of T cells loaded with HIV transactivator-peptide-derived superparamagnetic nanoparticles", J IMMUNOL M, 256(1-2), 2001, pp. 89-105

Abstract

The present study analyzed the feasibility of using magnetic resonance imaging (MRI) to monitor T-cell homing in vivo after loading T cells with superparamagnetic iron oxide (CLIO) nanoparticles derivatized with a peptide sequence from the transactivator protein (Tat) of HIV-1. T cells were isolated from C57BL/6 (B6) mice and loaded with 0, 400, 800, 1600, or 8000 ng/ml of FITC conjugated CLIO-Tat (FITC-CLIO-Tat). There was a dose-dependent uptake of FITC-CLIO-Tat by T cells. Stimulation of FITC-CLIO-Tat loaded T cellswith anti-CD3 (0.1 mug/ml) plus IL-2 (5 ng/ml) elicited normal activation and activation-induced cell death (AICD) responses, and normal upregulationof CD69, ICAM-1 (CD54), L-selectin (CD62L), and Fas. The FITC-CLIO-Tat loaded T cells (3 X 10(7)) were transferred intravenously (i.v.) into B6 mice and the in vivo MRI of mice was acquired using a spin-echo pulse sequence at 4.7 T with a Bruker Biospec system. Homing of T cells into the spleen wasobserved by a decrease in MRI signal intensity within I It after the transfer, which remained decreased for 2-24 h after transfer. These homing data were confirmed by FACS analysis and biodistribution analysis using I-125-CLIO-Tat. Thus, T cells can be efficiently loaded with FITC-CLIO-Tat without interfering with their normal activation and AICD, or homing to the spleen,and the biodistribution of FITC-CLIO-Tat loaded T cells can be monitored in vivo over time by MRI. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 09:30:54