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Titolo:
Rat liver slices as a tool to study LPS-induced inflammatory response in the liver
Autore:
Olinga, P; Merema, MT; de Jager, MH; Derks, F; Melgert, BN; Moshage, H; Slooff, MJH; Meijer, DKF; Poelstra, K; Groothuis, GMM;
Indirizzi:
Univ Groningen, Ctr Pharm, Dept Pharmacokinet & Drug Delivery, NL-9713 AV Groningen, Netherlands Univ Groningen Groningen Netherlands NL-9713 AV V Groningen, Netherlands Univ Groningen Hosp, Div Gastroenterol & Hepatol, Dept Internal Med, NL-9713 GZ Groningen, Netherlands Univ Groningen Hosp Groningen Netherlands NL-9713 GZ ningen, Netherlands Univ Groningen Hosp, Dept Surg, Div Hepatobiliary Surg & Liver Transplantat, NL-9713 GZ Groningen, Netherlands Univ Groningen Hosp Groningen Netherlands NL-9713 GZ ningen, Netherlands
Titolo Testata:
JOURNAL OF HEPATOLOGY
fascicolo: 2, volume: 35, anno: 2001,
pagine: 187 - 194
SICI:
0168-8278(200108)35:2<187:RLSAAT>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE SYNTHASE; TUMOR-NECROSIS-FACTOR; CYTOKINE GENE-EXPRESSION; IN-VITRO; KUPFFER CELLS; TISSUE-SLICES; HEPATOCYTES; LIPOPOLYSACCHARIDE; ENDOTOXIN; INTERLEUKIN-1-BETA;
Keywords:
cytokines; nitric oxide; iNOS; Kupffer cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Olinga, P Univ Groningen, Ctr Pharm, Dept Pharmacokinet & Drug Delivery, Ant Deusinglaan 1, NL-9713 AV Groningen, Netherlands Univ Groningen Ant Deusinglaan 1 Groningen Netherlands NL-9713 AV
Citazione:
P. Olinga et al., "Rat liver slices as a tool to study LPS-induced inflammatory response in the liver", J HEPATOL, 35(2), 2001, pp. 187-194

Abstract

Background/Aims: Inflammation in the liver is a complex interaction between parenchymal and non-parenchymal cells, and therefore can not be studied in vitro in pure cultures of these cells. Methods: We investigated whether Kupffer cells in the liver slice are still responsive to an inflammatory stimulus of lipopolysaccharide (LPS), and evoke an inflammatory response in the hepatocytes. Results: TNF alpha, IL-1 beta and IL-10 were significantly elevated in culture medium of LPS-stimulated rat liver slices. Nitric oxide (NO) production of LPS-treated slices gradually increased from 5 to 24 h (24 h: 81 +/- 5 muM vs. 14 +/- 2 muM in control P < 0.05), paralleled by inducible nitric oxide synthase (iNOS) in the hepatocytes, iNOS mRNA was induced after 3 h. NO production but not iNOS induction was significantly inhibited by NOS inhibitors S-methylisothiourea and N-G-nitro-L-arginine methylester. Both pentoxifylline and dexamethasone inhibited TNF alpha and IL-1 beta production, albeit to a different extent, iNOS induction and, as a result thereof, NO production. Conclusions: These results imply that non-parenchymal cells in liver slices are viable and can be activated by LPS. In addition, it is concluded thatthe upregulation of iNOS in hepatocytes by LPS is caused by cytokines produced by Kupffer cells because inhibition of TNF alpha and IL-1 beta production attenuated iNOS induction. (C) 2001 European Association for the Study of the Liver. Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 23:06:50