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Titolo:
Distribution of cocaine- and amphetamine-regulated transcript peptide in the guinea pig intrinsic cardiac nervous system and colocalization with neuropeptides or transmitter synthetic enzymes
Autore:
Calupca, MA; Locknar, SA; Zhang, LL; Harrison, TA; Hoover, DB; Parsons, RL;
Indirizzi:
Univ Vermont, Coll Med, Dept Anat & Neurobiol, Burlington, VT 05405 USA Univ Vermont Burlington VT USA 05405 Neurobiol, Burlington, VT 05405 USA E Tennessee State Univ, Dept Pharmacol, James H Quillen Coll Med, Johnson City, TN 37614 USA E Tennessee State Univ Johnson City TN USA 37614 hnson City, TN 37614 USA
Titolo Testata:
JOURNAL OF COMPARATIVE NEUROLOGY
fascicolo: 1, volume: 439, anno: 2001,
pagine: 73 - 86
SICI:
0021-9967(20011008)439:1<73:DOCAAT>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE SYNTHASE; GENE-RELATED PEPTIDE; SUBSTANCE-P; CHOLINE-ACETYLTRANSFERASE; HORSERADISH-PEROXIDASE; IMMUNOREACTIVE NERVES; ANTEROGRADE TRANSPORT; NADPH DIAPHORASE; AFFERENT-FIBERS; CART PEPTIDES;
Keywords:
CART; afferent fibers; intracardiac ganglia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Parsons, RL Univ Vermont, Coll Med, Dept Anat & Neurobiol, Burlington, VT 05405 USA Univ Vermont Burlington VT USA 05405 Burlington, VT 05405 USA
Citazione:
M.A. Calupca et al., "Distribution of cocaine- and amphetamine-regulated transcript peptide in the guinea pig intrinsic cardiac nervous system and colocalization with neuropeptides or transmitter synthetic enzymes", J COMP NEUR, 439(1), 2001, pp. 73-86

Abstract

This study was conducted to establish the presence of cocaine- and amphetamine-regulated transcript peptide (CARTp) immunoreactivity in neurons and fibers within guinea pig atrial whole-mount preparations containing the intrinsic cardiac ganglia. Many cardiac ganglia, but not all, in a given whole-mount preparation, were innervated by CARTp-immunoreactive (IR) fibers. Following explant culture of whole mounts for 72 hours, the CARTp-IR fiber networks were absent, but the number of CARTp-IR neurons was increased markedly. These observations suggested that the majority of the CARTp-IR fibers inthe intracardiac ganglia were derived from sources extrinsic to the heart. In control whole-mount preparations, very few CARTp-positive neurons were present. The few intrinsic CARTp-IR neurons also exhibited choline acetyltransferase (ChAT) immunoreactivity, indicating that they make up a small subpopulation of cholinergic postganglionic neurons. Some CARTp-IR neurons also exhibited nitric oxide synthase (NOS) immunoreactivity, indicating that they were nitrergic as well. We compared the immunohistochemical staining patterns of CARTp-IR fibers with the staining patterns of a number of other neurotransmitters or neurotransmitter synthetic enzymes that mark specific extrinsic inputs. The CARTp-IR fibers were not immunoreactive for ChAT, tyrosine hydroxylase, calcitonin gene-related peptide, or substance P. However,virtually all CARTp-IR fibers exhibited immunoreactivity to neuronal NOS (a marker for nitric oxide-producing neurons). CARTp-IR cells and NOS-IR cells were present in the nodose ganglia. In addition, CARTp-IR neurons in thenodose also were stained positively for NADPH-diaphorase. Thus, we proposethat most CARTp-IR fibers within the guinea. pig intrinsic cardiac gangliaare vagal afferent fibers that also contain NOS. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 04:34:48