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Titolo:
Expression of growth differentiation factor-15/macrophage inhibitory cytokine-1 (GDF-15/MIC-1) in the perinatal, adult, and injured rat brain
Autore:
Schober, A; Bottner, M; Strelau, J; Kinscherf, R; Bonaterra, GA; Barth, M; Schilling, L; Fairlie, WD; Breit, SN; Unsicker, K;
Indirizzi:
Univ Heidelberg, Neuroanat & Interdisciplinary Ctr Neurosci IZN, D-69120 Heidelberg, Germany Univ Heidelberg Heidelberg Germany D-69120 , D-69120 Heidelberg, Germany Univ Heidelberg, Fac Clin Med Mannheim, Devis Neurosurg Res, Dept Neurosurg, D-68135 Mannheim, Germany Univ Heidelberg Mannheim Germany D-68135 surg, D-68135 Mannheim, Germany St Vincents Hosp, Ctr Immunol, Sydney, NSW 2010, Australia St Vincents Hosp Sydney NSW Australia 2010 l, Sydney, NSW 2010, Australia
Titolo Testata:
JOURNAL OF COMPARATIVE NEUROLOGY
fascicolo: 1, volume: 439, anno: 2001,
pagine: 32 - 45
SICI:
0021-9967(20011008)439:1<32:EOGDFI>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
TGF-BETA SUPERFAMILY; MANGANESE SUPEROXIDE-DISMUTASE; NERVOUS-SYSTEM; TRANSFORMING GROWTH-FACTOR-BETA-1; MACROPHAGE ACTIVATION; OXIDIZED LDL; MEMBER; CELLS; MIC-1; IDENTIFICATION;
Keywords:
macrophages; microglia; choroid plexus; cortical lesion; TGF-beta superfamily member, CNS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Schober, A Univ Heidelberg, Neuroanat & Interdisciplinary Ctr Neurosci IZN, Neuenheimer Feld 307, D-69120 Heidelberg, Germany Univ Heidelberg Neuenheimer Feld 307 Heidelberg Germany D-69120
Citazione:
A. Schober et al., "Expression of growth differentiation factor-15/macrophage inhibitory cytokine-1 (GDF-15/MIC-1) in the perinatal, adult, and injured rat brain", J COMP NEUR, 439(1), 2001, pp. 32-45

Abstract

We and others have recently cloned a new member of the transforming growthfactor-fi superfamily, growth differentiation factor-15/ macrophage inhibitory cytokine-1 (GDF-15/MIC-1). Using in situ hybridization and immunohistochemistry, we determined the distribution of GDF-15/MIC-1 mRNA and protein in the perinatal and cryolesioned adult rat brain. The choroid plexus epithelium of all ventricles represents the site of strongest and almost exclusive mRNA expression in the normal perinatal and adult brain. The newborn ratbrain reveals GDF-15/MIC-1 immunoreactivity (ir) in ependymal cells liningthe ventricles, in the striatal subventricular zone, and in populations ofnonneural cells of the thalamic/hippocampal lamina affixa, in addition to that in the choroid plexus. Unilateral cryogenic cortical lesioning induceda significant increase of GDF-15/MIC-1 mRNA expression and ir at the lesion site and expression in presumed neurons within the dorsal thalamic area. At the lesion site, GDF-15/MIC-1-producing cells showed immuncytochemical features of neurons, macrophages, and activated microglial cells. Flourescent microscopy revealed both intra- and extracellular GDF-15/MIC-1 ir. Up-regulation of GDF-15/MIC-1 in activated macrophages (M phi) is also supported by RT-PCR, ICC, and Western blot experiments showing pronounced induction of GDF-15/MIC-1 expression (mRNA and protein) in retinoic acid/phorbol ester-stimulated human M phi. Our data suggest that 1) GDF-15/MIC-1 is secreted into the cerebrospinal fluid and 2) in the newborn brain may penetrate through the ependymal lining and act on developing neurons and/or glial cells. As a constituent of cells in the lamina affixa, the protein might be involved in the regulation of mesenchyme-epithelial interactions. Finally, GDF-15/MIC-1 may also act within the antiinflammatory cytokine network activated in CNS lesions. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 15:45:57