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Titolo:
Molecular typing of the etiologic agent of human granulocytic ehrlichiosis
Autore:
Carter, SE; Ravyn, MD; Xu, YN; Johnson, RC;
Indirizzi:
Univ Minnesota, Acad Hlth Ctr, Dept Microbiol, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 robiol, Minneapolis, MN 55455 USA
Titolo Testata:
JOURNAL OF CLINICAL MICROBIOLOGY
fascicolo: 9, volume: 39, anno: 2001,
pagine: 3398 - 3401
SICI:
0095-1137(200109)39:9<3398:MTOTEA>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
FRAGMENT-LENGTH-POLYMORPHISM; TICKS IXODES PACIFICUS; NEW-YORK-STATE; NORTHERN CALIFORNIA; LYME-DISEASE; BORRELIA-BURGDORFERI; SEQUENCE-ANALYSIS; HUMORAL RESPONSE; ENZOOTIC CYCLE; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Johnson, RC Univ Minnesota, Acad Hlth Ctr, Dept Microbiol, 420 Delaware StSE,Mayo Mail Code 196, Minneapolis, MN 55455 USA Univ Minnesota 420 Delaware St SE,Mayo Mail Code 196 Minneapolis MN USA 55455
Citazione:
S.E. Carter et al., "Molecular typing of the etiologic agent of human granulocytic ehrlichiosis", J CLIN MICR, 39(9), 2001, pp. 3398-3401

Abstract

The p44 gene of the agent of human granulocytic ehrlichiosis (aoHGE) encodes a 44-kDa major outer surface protein. A technique was developed for the typing of the aoHGE based on the PCR amplification of the p44 gene followedby a multiple restriction digest with HindIII, EcoRV, and AspI to generaterestriction fragment length polymorphism patterns. Twenty-four samples of the aoHGE were collected from geographically dispersed sites in the United States and included isolates from humans, equines, canines, small mammals, and ticks. Six granulocytic ehrlichiosis (GE) types were identified. The GEtyping method is relatively simple to perform, is reproducible, and is able to differentiate among the various isolates of granulocytic ehrlichiae inthe United States. These characteristics suggest that this GE typing method may be an important epizootiological and epidemiological tool.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 14:34:23