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Titolo:
PCR detection and molecular identification of Chlamydiaceae species
Autore:
Hartley, JC; Kaye, S; Stevenson, S; Bennett, J; Ridgway, G;
Indirizzi:
Univ Coll London Hosp NHS Trust, Dept Clin Microbiol, London WC1E 6DB, England Univ Coll London Hosp NHS Trust London England WC1E 6DB C1E 6DB, England Royal Free & Univ Coll Med Sch, Dept Virol, London W1T 4JF, England Royal Free & Univ Coll Med Sch London England W1T 4JF n W1T 4JF, England
Titolo Testata:
JOURNAL OF CLINICAL MICROBIOLOGY
fascicolo: 9, volume: 39, anno: 2001,
pagine: 3072 - 3079
SICI:
0095-1137(200109)39:9<3072:PDAMIO>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
PNEUMONIAE INFECTION; FAMILY CHLAMYDIACEAE; ORDER CHLAMYDIALES; ANTIBODIES; PSITTACI; DIAGNOSIS; STRAINS; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Hartley, JC Great Ormond St Hosp Children NHS Trust, Camelia Botnar Labs, Dept Microbiol, Level 4,Great Ormond St, London WC1N 3JH, England Great Ormond St Hosp Children NHS Trust Level 4,Great Ormond St London England WC1N3JH
Citazione:
J.C. Hartley et al., "PCR detection and molecular identification of Chlamydiaceae species", J CLIN MICR, 39(9), 2001, pp. 3072-3079

Abstract

Recent taxonomic developments, based on 16s and 23s rRNA gene sequences, have divided the family Chlamydiaceae into two genera and nine species, of which five have been found to infect humans. Few simple methods are available to detect and identify all species sensitively and specifically. In this study the suitability of the omp2 gene ag a target for molecular identification of Chlamydiaceae is demonstrated. Phylogenetic analysis of partial omp2 gene sequences from all nine species agrees with the recently published taxonomic changes based on the ribosomal genes. The use of a family-specificPCR primer pair, which is able to amplify the 5' end of the omp2 gene fromall Chlamydiaceae except some Chlamydophila pecorum strains, is described. Identification of all nine species was achieved using restriction fragmentlength polymorphism analysis with a single enzyme, AluI confirmed by DNA sequencing. A PCR enzyme-linked oligonucleotide assay was developed which can detect a single chlamydial genome and may be applied to DNA extracts fromany specimen or culture for the detection of single or mixed human chlamydial infection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/01/21 alle ore 23:18:54