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Titolo:
Low-denisty caveolae-like membrane from Xenopus laevis oocytes in enrichedin Ras
Autore:
Sadler, SE;
Indirizzi:
Univ Denver, Dept Biol Sci, Denver, CO 80208 USA Univ Denver Denver CO USA 80208 nver, Dept Biol Sci, Denver, CO 80208 USA
Titolo Testata:
JOURNAL OF CELLULAR BIOCHEMISTRY
fascicolo: 1, volume: 83, anno: 2001,
pagine: 21 - 32
SICI:
0730-2312(2001)83:1<21:LCMFXL>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR-MEDIATED ENDOCYTOSIS; GROWTH FACTOR-I; 1ST MEIOTIC DIVISION; PLASMA-MEMBRANE; AMPHIBIAN OOCYTE; ADENYLATE-CYCLASE; GENE FAMILY; CELL-CYCLE; INSULIN; PROGESTERONE;
Keywords:
Xenopus laevis; oocytes; caveolae; caveolin; low-density membrane; Ras;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Sadler, SE Univ Denver, Dept Biol Sci, Univ Pk, Denver, CO 80208 USA Univ Denver Univ Pk Denver CO USA 80208 k, Denver, CO 80208 USA
Citazione:
S.E. Sadler, "Low-denisty caveolae-like membrane from Xenopus laevis oocytes in enrichedin Ras", J CELL BIOC, 83(1), 2001, pp. 21-32

Abstract

Detergent-free discontinuous sucrose density gradient centrifugation was used to resolve low- and high-density membrane fractions from Xenopus laevisoocytes. Compared to high-density membrane, low-density oocyte membrane isenriched two-fold in cholesterol and highly enriched in ganglioside GM1. Protein immunoblotting of membrane fractions from whole ce] Is with polyclonal anti-human caveolin antibody detected multiple bands, including a distinctive triad with apparent molecular weights of 21, 33, and 48 kDa. To more clearly determine which of these caveolin-like protein(s) is associated with the oocyte plasma membrane, microdissection was used to separate externalmembrane (cortical preparations containing plasma membrane) from intracellular membrane. Cortical membrane preparations displayed a single 21-kDa caveolin-like protein in low-density membrane. Internal oocyte membrane displayed the higher molecular weight bands of 33 and 48 kDa and a lesser amount of the 21-kDa protein in low-density membrane fractions. Monoclonal anti-human Ras antibody detected a single 23-kDa immunoblot band that is enriched an average of eight-fold in low-density membrane fractions prepared from whole cells. This is the first report of caveolin-associated, low-density membrane in amphibian oocytes, and is consistent with a role for caveolin and caveolae-like microdomains in oocyte signal transduction. J. Cell. Biochem.83: 21 -32, 2001. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 16:17:32