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Titolo:
The promoter of mouse transcription repressor bach1 is regulated by Sp1 and trans-activated by Bach1
Autore:
Sun, JY; Muto, A; Hoshino, H; Kobayashi, A; Nishimura, S; Yamamoto, M; Hayashi, N; Ito, E; Igarashi, K;
Indirizzi:
Hiroshima Univ, Sch Med, Dept Biochem, Hiroshima 7348551, Japan Hiroshima Univ Hiroshima Japan 7348551 Biochem, Hiroshima 7348551, Japan Tohoku Univ, Sch Med, Dept Biochem, Sendai, Miyagi 9808575, Japan Tohoku Univ Sendai Miyagi Japan 9808575 em, Sendai, Miyagi 9808575, Japan Univ Tsukuba, Ctr Tsukuba Adv Res Alliance, Tsukuba, Ibaraki 3058577, Japan Univ Tsukuba Tsukuba Ibaraki Japan 3058577 sukuba, Ibaraki 3058577, Japan Univ Tsukuba, Inst Basic Med Sci, Tsukuba, Ibaraki 3058577, Japan Univ Tsukuba Tsukuba Ibaraki Japan 3058577 sukuba, Ibaraki 3058577, Japan Hirosaki Univ, Sch Med, Dept Pediat, Hirosaki, Aomori 036, Japan Hirosaki Univ Hirosaki Aomori Japan 036 diat, Hirosaki, Aomori 036, Japan
Titolo Testata:
JOURNAL OF BIOCHEMISTRY
fascicolo: 3, volume: 130, anno: 2001,
pagine: 385 - 392
SICI:
0021-924X(200109)130:3<385:TPOMTR>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMALL MAF PROTEINS; LOCUS-CONTROL REGION; BTB/POZ DOMAIN; FACTOR NF-E2; MONOCYTIC DIFFERENTIATION; MOLECULAR-CLONING; GENE-EXPRESSION; MUTANT MICE; FAMILY; ONCOPROTEIN;
Keywords:
Maf; oncogene; promoter; transcription factor; transcription repression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Igarashi, K Hiroshima Univ, Sch Med, Dept Biochem, Kasumi 1-2-3, Hiroshima7348551, Japan Hiroshima Univ Kasumi 1-2-3 Hiroshima Japan 7348551 51, Japan
Citazione:
J.Y. Sun et al., "The promoter of mouse transcription repressor bach1 is regulated by Sp1 and trans-activated by Bach1", J BIOCHEM, 130(3), 2001, pp. 385-392

Abstract

The Maf recognition element (MARE) is regulated by both activators and repressors. Bach1 and Bach2 repress MARE-dependent transcription by forming heterodimers with Maf-related oncoproteins. In order to gain an understandingof the regulation of bach1 gene expression, we analyzed the structure of the mouse bach1 gene. Comparison of the exon-intron structure of the bach1 gene with those of other NF-E2-related genes indicated that bach1 and bach2 constitute an evolutionarily distinct subfamily among bZip factors. The bach1 promoter region contains two GC boxes that are important for its basal activity and are bound by Sp1 in K562 cell extracts. In addition, we found an evolutionarily conserved MARE-like element located downstream of the transcription initiation site. Deletion of this element resulted in a higher promoter activity in K562 cells. Bach1 trans-activated its own promoter depending on the presence of the MARE-like element in co-transfection assays. However, Bach1 did not bind to the MARE-like element in electrophoretic mobility shift assays (EMSA). These results suggest that Bach1 activates its ownpromoter indirectly by inhibiting the putative repressor. Such a positive feedback regulation by the repressor Bach1 may play an important role in maintaining the expression of Bach1 while consolidating repression of other genes with MARE.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/10/20 alle ore 21:25:38