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Titolo:
A method for micrometer resolution patterning of primary culture neurons for SPM analysis
Autore:
Degenaar, P; Le Pioufle, B; Griscom, L; Tixier, A; Akagi, Y; Morita, Y; Murakami, Y; Yokoyama, K; Fujita, H; Tamiya, E;
Indirizzi:
Japan Adv Inst Sci & Technol, Sch Mat Sci, Hokuri Ku, Tatsunokuchi, Ishikawa 9231292, Japan Japan Adv Inst Sci & Technol Tatsunokuchi Ishikawa Japan9231292 2, Japan Univ Tokyo, Inst Ind Sci, CNRS, LIMMS,Minato Ku, Tokyo 1068558, Japan UnivTokyo Tokyo Japan 1068558 RS, LIMMS,Minato Ku, Tokyo 1068558, Japan Ecole Normale Super, F-35170 Bruz, France Ecole Normale Super Bruz France F-35170 male Super, F-35170 Bruz, France
Titolo Testata:
JOURNAL OF BIOCHEMISTRY
fascicolo: 3, volume: 130, anno: 2001,
pagine: 367 - 376
SICI:
0021-924X(200109)130:3<367:AMFMRP>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-ADHESION; MICROSCOPE; PROTEINS; SYSTEM; FILMS;
Keywords:
neuron; patterning; SPM; SNOM; SNOAM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Degenaar, P Japan Adv Inst Sci & Technol, Sch Mat Sci, Hokuri Ku, 1-1 Asahidai, Tatsunokuchi, Ishikawa 9231292, Japan Japan Adv Inst Sci & Technol 1-1 Asahidai Tatsunokuchi Ishikawa Japan 9231292
Citazione:
P. Degenaar et al., "A method for micrometer resolution patterning of primary culture neurons for SPM analysis", J BIOCHEM, 130(3), 2001, pp. 367-376

Abstract

In this work we present a method for ultra-fine patterning of primary culture neuron cell growth, which is compatible for scanning near-field opticalatomic force microscopy (SNOAM) analysis. SNOAM uses near-field optics to break the fundamental diffraction limit imposed on normal microscopy. SNOAMcan achieve sub-100 nm optical resolutions, but requires transparent, opensubstrates. The ability to do physiological measurements on patterns of neurons, combined with ultra high resolution optical and fluorescent analysis, is useful in the study of long-term potentiation. The patterning method consists of chemical guidance with an element of physical confinement and allows for ultra-fine patterning of neural growth on transparent glass substrates. Substrates consist of microfabricated perfluoropolymer barrier structures on glass. Poly-L-lysine was selectively deposited using a silicone-based microfluidic stencil aligned to the perfluoropolymer/glass substrate. Primary culture neurons were extracted from 8-day-old chicks and grown for 3 days to form good networks. This patterning system shows very specific growth with patterning separations down to the level of individual neurites. Fluorescent imaging was carried out on both cell viability during growth and immuno-tagged microtubule-associated proteins on the neurites. Neurons inside the patterned structures were imaged and analyzed with a tapping mode SNOAM.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 02:22:56