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Titolo:
Interaction of endonuclease EcoRI with short specific and nonspecific oligonucleotides
Autore:
Kolocheva, TI; Maksakova, GA; Bugreev, DD; Nevinsky, GA;
Indirizzi:
Russian Acad Sci, Novosibirsk Bioorgan Chem Inst, Siberian Div, Novosibirsk 630090, Russia Russian Acad Sci Novosibirsk Russia 630090 v, Novosibirsk 630090, Russia
Titolo Testata:
IUBMB LIFE
fascicolo: 3, volume: 51, anno: 2001,
pagine: 189 - 195
SICI:
1521-6543(200103)51:3<189:IOEEWS>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-POLYMERASES; CATALYTIC PROPERTIES; HOMOGENEOUS ENZYME; RI ENDONUCLEASE; RESTRICTION; SEQUENCES; AFFINITY; RECOGNITION; METHYLASE; MECHANISM;
Keywords:
DNA recognition; EcoRI endonuclease; mechanism; non-specific interactions; specific;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Nevinsky, GA Russian Acad Sci, Novosibirsk Bioorgan Chem Inst, Siberian Div, LavrentievAve 8, Novosibirsk 630090, Russia Russian Acad Sci Lavrentiev Ave 8 Novosibirsk Russia 630090
Citazione:
T.I. Kolocheva et al., "Interaction of endonuclease EcoRI with short specific and nonspecific oligonucleotides", IUBMB LIFE, 51(3), 2001, pp. 189-195

Abstract

The interaction of EcoRI with different oligodeoxyribonucleotides (ODNs) was analyzed using the method of the slow step-by-step simplification in their complexity. Orthophosphate (K-I = 31 mM), 2-deoxyribose 5-phosphate (K-I= 4.6 mM) and different dN-MPs (K-I = 2.1-2.5 mM) were shown to be the minimal ligands of the enzyme. The lengthening of a nonspecific d(pN)(n) (n = 1-6) by one nucleotide unit resulted in the increase of their affinity by afactor of similar to2.0. Weak nonspecific electrostatic contacts of EcoRI with internucleotide phosphate groups of ODNs can account for about 5 orders of magnitude in the ligand affinity, whereas the contribution of specificinteractions between EcoRI and d(pN), is no more than 2 orders of magnitude of a total ODN's affinity.

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Documento generato il 02/04/20 alle ore 11:54:20