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Titolo:
Morphology and neurochemistry of canine corneal innervation
Autore:
Marfurt, CF; Murphy, CJ; Florczak, AL;
Indirizzi:
Indiana Univ, Sch Med, NW Ctr Med Educ, Dept Anat & Cell Biol, Gary, IN 46408 USA Indiana Univ Gary IN USA 46408 Dept Anat & Cell Biol, Gary, IN 46408 USA Univ Wisconsin, Sch Vet Med, Dept Surg Sci, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 Dept Surg Sci, Madison, WI 53706 USA
Titolo Testata:
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
fascicolo: 10, volume: 42, anno: 2001,
pagine: 2242 - 2251
SICI:
0146-0404(200109)42:10<2242:MANOCC>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUPERIOR CERVICAL-GANGLION; GENE-RELATED PEPTIDE; GROWTH-FACTOR-I; SUBSTANCE-P; EPITHELIAL-CELLS; IMMUNOHISTOCHEMICAL LOCALIZATION; SYMPATHETIC-STIMULATION; IMMUNOREACTIVE NERVES; TYROSINE-HYDROXYLASE; CILIARY GANGLION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Marfurt, CF Indiana Univ, Sch Med, NW Ctr Med Educ, Dept Anat & Cell Biol,3400 Broadway, Gary, IN 46408 USA Indiana Univ 3400 Broadway Gary IN USA 46408 ary, IN 46408 USA
Citazione:
C.F. Marfurt et al., "Morphology and neurochemistry of canine corneal innervation", INV OPHTH V, 42(10), 2001, pp. 2242-2251

Abstract

PURPOSE. To determine the architectural pattern and neuropeptide content of canine corneal innervation. METHODS. Corneal nerve fibers in normal dog eyes were labeled immunohistochemically with antibodies against protein gene product (PGP)-9.5, calcitonin gene-related peptide (CGRP), substance P (SP), vasoactive intestinal polypeptide (VI-P), and tyrosine hydroxylase (TH). Relative innervation densities and distribution patterns for each fiber population were assessed qualitatively by serial line-drawing reconstructions and quantitatively by computer-assisted analyses. RESULTS. More than 99% of all corneal PGP-9.5-immunoreactive (IR) nerves contained both CGRP and SP, approximately 30% contained TH, and none contained VIP. Distribution patterns of corneal PGP-9.5-, CGRP-, SP-, and TH-IR nerves were indistinguishable, except that TH-IR fibers were absent from the corneal epithelium. Morphologically, canine corneal innervation consisted of a: rich anterior stromal. plexus, divided on the basis of morphologic, criteria into anterior and posterior levels, and a rich epithelial innervation, characterized by large numbers of horizontally oriented, basal epithelial "leash" formations. Leash axons in all quadrants of the corneal epithelium oriented preferentially toward a common locus in the perilimbal cornea. CONCLUSIONS. The results of this study demonstrate for the first time the detailed architectural features, distinctive basal epithelial leash orientations, and peptidergic content of canine corneal innervation. The normal innervation pattern described in this study will provide other investigators with essential baseline data for assessing corneal nerve alterations in canine patients with spontaneous chronic corneal epithelial defects (SCCED) and other ocular diseases or injuries.

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Documento generato il 11/07/20 alle ore 21:10:19