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Titolo:
Interaction between 52 kDa SSA/Ro and deubiquitinating enzyme UnpEL: a clue to function
Autore:
Di Donato, F; Chan, EKL; Askanase, AD; Miranda-Carus, ME; Buyon, JP;
Indirizzi:
NYU, Hosp Joint Dis, Sch Med, Dept Rheumatol, New York, NY 10003 USA NYU New York NY USA 10003 Sch Med, Dept Rheumatol, New York, NY 10003 USA Scripps Clin & Res Inst, La Jolla, CA USA Scripps Clin & Res Inst La Jolla CA USA lin & Res Inst, La Jolla, CA USA
Titolo Testata:
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
fascicolo: 9, volume: 33, anno: 2001,
pagine: 924 - 934
SICI:
1357-2725(200109)33:9<924:IB5KSA>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONGENITAL HEART-BLOCK; NEONATAL LUPUS-ERYTHEMATOSUS; PROTEIN PROTEIN INTERACTIONS; SS-A/RO AUTOANTIGEN; INTRACELLULAR-LOCALIZATION; CONDUCTION ABNORMALITIES; MURINE MODEL; RING FINGER; RO/SS-A; RO RNAS;
Keywords:
congenital heart block; deubiquitinating enzyme; neonatal lupus; RING finger; SSA/Ro antibody; ubiquitin; UnpEL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Buyon, JP NYU, Hosp Joint Dis, Sch Med, Dept Rheumatol, Room 1608,301 E 17th St, NewYork, NY 10003 USA NYU Room 1608,301 E 17th St New York NY USA 10003 , NY 10003 USA
Citazione:
F. Di Donato et al., "Interaction between 52 kDa SSA/Ro and deubiquitinating enzyme UnpEL: a clue to function", INT J BIO C, 33(9), 2001, pp. 924-934

Abstract

The detection of isolated heart block in utero strongly predicts the presence of maternal autoantibodies reactive with 52 kDa. SSA/Ro. The mechanismsthat underlie this observation may be elucidated by defining the function of the target antigen. The initial approach was to identify proteins interactive with 52Ro using transcriptional activity in the yeast 2-hybrid system. A cDNA library was constructed using RNA isolated from human fetal hearts(12-23 weeks) and cloned into the HybriZAP vector encoding the activation domain of GAL4(AD) as target. Approximately 7 x 10(6) cDNAs were cotransformed with the bait into YRG-2. Plasmids from five interactive colonies were sequenced and three identified as the specific human deubiquitinating enzyme, UnpEL. UnpEL did not interact with bait plasmid encoding 52 beta, an alternative leucine zipper-minus form of 52 kDa SSA/Ro which is maximally expressed in fetal life. The mammalian 2-hybrid assay confirmed the interactionbetween full-length 52Ro and UnpEL. Further support for a biologic interaction was the marked redistribution in cellular localization of UnpEL following cotransfection of the two proteins into cultured human cardiocytes, human renal carcinoma cells (293 cells), and monkey kidney fibroblasts (COS-I). In conclusion, the interaction of full-length 52Ro and UnpEL implies thatthe former may also be involved in the ubiquitin pathway, an observation of particular interest since 52Ro contains a RING finger domain, a motif common to several recently reported proteins involved in modulating ubiquitination. The absence of an interaction with 52 beta raises the consideration that regulation of protein ubiquitination might differ in fetal life. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 16:09:33